<p>To evaluate the effect of dietary supplementation of DL-methionine and L-lysine in hypoprotein diets on productivity and egg quality, a total of 240 White Leghorn laying hens (Hybrid L-33) of 26 weeks during laying peak period were placed for 49 days, according to completely randomized design with four treatments and 10 repetitions. Dietary treatments consisted of a control diet (no amino acid supplementation) (T0) and supplementation of 0.03% DL-Met and 0.04% L-Lys (T1); 0.06% DL-Met and 0.08% L-Lys (T2) and 0.09% DL-Met and 0.12% L-Lys (T3). At 26 and 33 weeks of age, 30 eggs per treatment were collected to determine internal and external egg quality parameters.</p><p>The experiment diets did not affect the viability and feed intake. Supplementation of essential amino acids, especially the T3 improved egg weight (54.30 to 58.30 g), laying rate (76.89 to 85.45%) and mass conversion (2.52 to 2.11 kg/kg) (P&lt;0.05). At 33 week, the albumin height (7.21 to 8.23 mm), Haugh units (86.70 to 91.15) (P&lt;0.05) and shell surface (67.10 to 69.20 cm2) increased by the effect of supplementation of DL-Met and L-Lys. The results allow recommending the dietary supplementation of 0.09% DL-Met and 0.12% L-Lys in hypoprotein diets to improve productivity and egg quality in laying hens.</p>

With the objective of determining the nutritional constituents, amino acids and the content of secondary metabolites of the foliage meal of Tithonia diversifolia. For this, plant material was collected 70 days after regrowth. The percentages of DM, CP, P, Ca, Si, CF, NDF, ADF, ADL, Cel, Hcel, CC were determined; amino acid profile (methionine, cystine, methionine + cystine, lysine, threonine, aspartic acid, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, serine, phenylalanine, arginine, histidine), and content of TT, TP, TCT, TBCT, FCT, Flv, Sap, Alk, Trit and TS. For statistical processing, descriptive techniques (mean and standard deviation) were used. The plant material of Tithonia diversifolia analyzed showed percentages of 22.23, 2.62, 0.013, 45.7, and 29.8% and 6.09 MJ/kg (CP, Ca, P, NDF, ADF and ME). For the secondary metabolites, they presented concentrations of 5.35, 12.38, 13.7, 9.58, 4.15, 24.49, 0.86, 1.36, 7.71 and 10.75 g/kg MS for total tannins, total phenols, total condensed tannins, total bound condensed tannins, free condensed tannins, flavonoids, alkaloids, saponins, triterpenes and total steroids). The highest concentrations of amino acids were for lysine, aspartic acid, glutamic acid, proline, glycine, alanine, valine, leucine, serine and phenylalanine with values ​​of 13-30 g/kg of protein. It is concluded that the foliage meal of Tithonia diversifolia presents an adequate relation in its quality.