In searching for persistent seizure-induced alterations in brain function that might be causally related to epilepsy, presynaptic transmitter release has relatively been neglected. To measure directly the long-term effects of pilocarpine-induced status epilepticus on vesicular release and recycling in hippocampal mossy fibre presynaptic boutons, we used (i) two-photon imaging of FM1-43 vesicular release in rat hippocampal slices; and (ii) transgenic mice expressing the genetically encoded pH-sensitive fluorescent reporter synaptopHluorin preferentially at glutamatergic synapses. In this study we found that, 1-2 months after pilocarpine-induced status epilepticus, there were significant increases in mossy fibre bouton size, faster rates of action potential-driven vesicular release and endocytosis. We also analysed the ultrastructure of rat mossy fibre boutons using transmission electron microscopy. Pilocarpine-induced status epilepticus led to a significant increase in the number of release sites, active zone length, postsynaptic density area and number of vesicles in the readily releasable and recycling pools, all correlated with increased release probability. Our data show that presynaptic release machinery is persistently altered in structure and function by status epilepticus, which could contribute to the development of the chronic epileptic state and may represent a potential new target for antiepileptic therapies.

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The interest to decrease the complications and morbility in patients, encourage the investigation to search for minor invasvies surgical techniques. The medical responsability for the wellness of the patient adds to the actual advanced technologies that is the origin to propose more efficent interventions. An example is the XLIF (extreme laterla interbody fusion) represents one of the avant-gard techniques of spine surgery. The XLIF represents an excelent option for the treatment of some spine compelx pathologies that needs descompression, balance alignement, arthrodesis and stabilization: degenerative disc disease, spondylolisthesis grade l and ll, deformities like degenerative scoliosis or adjacent disc disease are examples where the benefit of this technicc is evident. The XLIF represents an excelent option for the treatment of some spine compelx pathologies that needs descompression, balance alignement, arthrodesis and stabilization: degenerative disc disease, spondylolisthesis grade l and ll, deformities like degenerative scoliosis or adjacent disc disease are examples where the benefit of this technicc is evident. However, this method is contraindicated for L5-S1 (limited by iliac crest) disc disease or spondylolisthesis grade lll for example.