In this paper the results of a field investigation on rilling carried out in the experimental Sparacia area are reported.The measurements were made on a plot 6 m wide and 22 m long subjected to natural rainfalls. For ten rainfalls the total soil loss (interrill and rill erosion) was collected in a storage system consisting of two tanks arranged in series at the base of the plot. Rill morphology (rill length and cross-sections) was measured for five rainfall events, while the rill profile was surveyed for three events.First the contribution of each component (rill and interrill erosion) to total soil loss was established. Then the analysis allowed establishment of a power relationship between the rill length and the rill volume. Finally, for three events detailed information on rill erosion and rill morphology allowed verification of the applicability of WEPP and estimation of the rill erodibility constant. Figure 12. Comparison between Equation (2) and the pairs (L, V ) available for five erosive events.Figure 13. Rill cross-sections.Figure 15. Comparison between Equation (1) and the pairs (τ − τ c , D r ).

Medical devices, such as silicone-based prostheses designed for soft tissue implantation, often induce a suboptimal foreign-body response which results in a hardened avascular fibrotic capsule around the device, often leading to patient discomfort or implant failure. Here, it is proposed that additive manufacturing techniques can be used to deposit durable coatings with multiscale porosity on soft tissue implant surfaces to promote optimal tissue integration. Specifically, the "liquid rope coil effect", is exploited via direct ink writing, to create a controlled macro open-pore architecture, including over highly curved surfaces, while adapting atomizing spray deposition of a silicone ink to create a microporous texture. The potential to tailor the degree of tissue integration and vascularization using these fabrication techniques is demonstrated through subdermal and submuscular implantation studies in rodent and porcine models respectively, illustrating the implant coating's potential applications in both traditional soft tissue prosthetics and active drug-eluting devices.

Adipose derived microvascular fragments (ad-MVFs) consist of effective vascularization units able to reassemble into efficient microvascular networks. Because of their content in stem cells and related angiogenic activity, ad-MVFs represent an interesting tool for applications in regenerative medicine. Here we show that gentle dissociation of rat adipose tissue provides a mixture of ad-MVFs with a length distribution ranging from 33–955 μm that are able to maintain their original morphology. The isolated units of ad-MVFs that resulted were able to activate transcriptional switching toward angiogenesis, forming tubes, branches, and entire capillary networks when cultured in 3D collagen type-I hydrogel. The proper involvement of metalloproteases (MMP2/MMP9) and serine proteases in basal lamina and extracellular matrix ECM degradation during the angiogenesis were concurrently assessed by the evaluation of alpha-smooth muscle actin (αSMA) expression. These results suggest that collagen type-I hydrogel provides an adequate 3D environment supporting the activation of the vascularization process. As a proof of concept, we exploited 3D collagen hydrogel for the setting of ad-MVF–islet of Langerhans coculture to improve the islets vascularization. Our results suggest potential employment of the proposed in vitro system for regenerative medicine applications, such as the improving of the islet of Langerhans engraftment before transplantation.

Chondrocyte transplantation has been successfully tested and proposed as a clinical procedure aiming to repair articular cartilage defects. However, the isolation of chondrocytes and the optimization of the enzymatic digestion process, as well as their successful in vitro expansion, remain the main challenges in cartilage tissue engineering. In order to address these issues, we investigated the performance of recombinant collagenases in tissue dissociation assays with the aim of isolating chondrocytes from bovine nasal cartilage in order to establish the optimal enzyme blend to ensure the best outcomes of the overall procedure. We show, for the first time, that collagenase H activity alone is required for effective cartilage digestion, resulting in an improvement in the yield of viable cells. The extracted chondrocytes proved able to grow and activate differentiation/dedifferentiation programs, as assessed by morphological and gene expression analyses.

In article number 2100229 by Eoin O'Cearbhaill, Garry Duffy and co‐workers, the “liquid rope coil effect” is harnessed to produce porous silicone coatings that are additively manufactured onto the surface of soft‐tissue implants, aiming to address notorious challenges of long‐term tissue integration and fibrosis associated with these implants. In vivo studies show that this coating encourages highly vascularised tissue ingrowth