RESUMEN.-La lombriz de tierra, Eisenia foetida, es hospedero de numerosos microorganismos simbiontes, muchos de los cuales han sido identificados recientemente, así como se ha descrito su actividad enzimática. Estos microorganismos suelen presentar actividad enzimática de diferente tipo. Algunos podrían presentar interés industrial por sus potenciales aplicaciones. En el presente trabajo, se eligió identificar aquellos microorganismos que tienen actividad endo-β-1,4-glucanasa, y evaluar dicha actividad por el método de los azúcares reductores de Nelson-Somogyi. Dichos azúcares reductores fueron liberados después de la incubación de las bacterias con la solución de carboximetilcelulosa (CMC). Las colonias aisladas de interés fueron cultivadas en Plate Count Agar, PCA (Difco), y su identificación se llevó a cabo mediante el análisis de la región 16SDNAr. Comparando los resultados con la base de datos NCBI, se encontró que las especies productoras de la actividad endo-β-1,4-glucanasa fueron: Burkholderia fhytofirmans, Bacillus amyloliquefaciens, Bacillus licheniformis, y Acidovorax delafieldii. Los aislamientos produjeron un porcentaje de degradación interesante en relación al control positivo empleado. Se presentan comparaciones de la degradación causada por las diferentes colonias aisladas. PALABRAS CLAVE:Endo-β-1,4-glucanasa, enzimas, lombriz de tierra, microbiota, 16SDNAr.ABSTRACT.-The earth worm, Eisenia foetida, is host for numerous symbiotic microorganisms, many of which have been recently identified, as well as the description of their enzymatic activity. These microorganisms usually present enzymatic activity of different type, and some may present industrial interest due to their potential applications. In the present research, it was decided to identify those microorganisms that present endo-β-1,4-glucanasa activity, and to evaluate this activity by the Nelson-Somogyi method of reducing sugars. Reducing sugars were released after the incubation of the bacteria colonies with carboxymethyl cellulose (CMC) solution. The isolated colonies of interest were cultured in laboratory and their identification was made through analysis of the 16S DNAr region. By comparing the results with the NCBI database, it was found that the endo-β-1,4-glucanase activity producing species were: Burkholderia fhytofirmans, Bacillus amyloliquefaciens, Bacillus licheniformis, and Acidovorax delafieldii. The isolations produced an interesting degradation percentage in relation to the positive control utilized. Comparisons of the degradation caused by the different isolated colonies are presented.
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