The electrochemical characterization of a hydrogen peroxide sensor based on a ferrocene-containing polymer electrochemically deposited onto a platinum electrode is described. The redox polymer consists of a siloxane-based homopolymer, with pendant electronically communicated ferrocenyl moieties. The electrodes were used as the transducer for glucose and lactate-sensing enzyme sensors. Amperometric biosensors were prepared by immobilization of glucose oxidase (Gox) or lactate oxidase (Lox) onto these modified electrodes. The steady-state amperometric response of the sensors is investigated as a function of the applied potential and substrate concentration. Interferences, sensitivity and stability of the sensors were also studied.
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