The present study aimed to evaluate the seroprevalence of anti - Brucella abortus antibodies in Veterinary Medicine students at the Federal University of Mato Grosso (UFMT) - Sinop campus and Veterinary Physicians in the mid-north region of Mato Grosso, as well as the risk factors associated with its transmission. Blood samples were collected from the participants to assess the frequency of anti-Brucella abortus antibodies and sent to the Infectious Diseases Laboratory at UFMT Campus Sinop, where the samples were processed. The blood samples collected from indirect diagnostic methods, based on the detection of antibodies. This is an observational, cross-sectional, retrospective study, in which the results were evaluated through the analysis of the chi-square association by Fisher's exact test and for the identification of factors associated with the reagents for anti-Brucella abortus, it was carried out crude analysis using Poisson regression. Of the 213 volunteers, seven were tested for the Buffered Acidified Antigen (42.9%) and with a predominant range of age from 18 to 25 years (42.9%) %). The variable “Frequent Laboratory that diagnoses Brucellosis” (p = 0.028) was the only variable that remained significant after regression. It was concluded the lack of preparation and responsibility of some professionals when exercising the profession, not fulfilling the basics: protecting themselves and helping others to do the same, putting their health at risk and that of the population in general.