Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen associated with disease in a range of domestic and wild animals. Despite its importance, very limited data is available on its survival and persistence on the environment. The goal of this study was to evaluate survival of S. zooepidemicus under ideal culture conditions and farm-like setting, in various surface types. Rubber, plastic, wood, and concrete samples were sterilized and inoculated with 109 CFU/mL of S. zooepidemicus with or without feces, and cultured under ideal conditions (37 °C, 5% CO2) or farm-like settings (20oC on air) for a maximum period of 25 days (n = 3/material/environment/feces-group/time-point). Under ideal conditions without feces, the bacterium survived for up to 17 days on plastic and rubber surfaces, 4 days on wood and less than 1 day on concrete (P < 0.05 between materials). Samples under ideal conditions with feces and farm-like settings without feces were negative by day 1 post-inoculation, regardless of the surface material used. Wood and concrete allowed S. zooepidemicus persistence for up to 3 days under farm-like settings when feces were present. This data suggests that environmental persistence of S. zooepidemicus is affected by surface type and incubation temperature.