A receptor binding assay (RBA) for detection of paralytic shellfish poisoning (PSP) toxins was formatted for use in a high throughput detection system using microplate scintillation counting. The RBA technology was transferred from the National Ocean Service, which uses a Wallac TriLux 1450 MicroBeta microplate scintillation counter, to the California Department of Health Services, which uses a Packard TopCount scintillation counter. Due to differences in the detector arrangement between these 2 counters, markedly different counting efficiencies were exhibited, requiring optimization of the RBA protocol for the TopCount instrument. Precision, accuracy, and sensitivity [limit of detection = 0.2 μg saxitoxin (STX) equiv/100 g shellfish tissue] of the modified protocol were equivalent to those of the original protocol. The RBA robustness and adaptability were demonstrated by an interlaboratory study, in which STX concentrations in shellfish generated by the TopCount were consistent with MicroBeta-derived values. Comparison of STX reference standards obtained from the U.S. Food and Drug Administration and the National Research Council, Canada, showed no observable differences. This study confirms the RBA's value as a rapid, high throughput screen prior to testing by the conventional mouse bioassay (MBA) and its suitability for providing an early warning of increasing PSP toxicity when toxin levels are below the MBA limit of detection.

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Whether the uranium MCL is met in California depends on what conversion factor is used. Uranium, a kidney toxicant and a radioactive material, is present at elevated concentrations in groundwater in some states. In 1991 the US Environmental Protection Agency proposed a maximum contaminant level (MCL) for uranium in drinking water of 20 μg/L or 30 pCi/L (1.1 Bq/L). In 1992 California adopted an MCL of 20 pCi/L (0.74 Bq/L). Because California regulations permit analysis of uranium by either mass or radiochemical techniques, a reliable factor that converts values from mass to activity is required to determine compliance with the California MCL and any future federal MCL. The conversion factor for groundwater may differ markedly from that of the natural crustal abundance of uranium. This study compared values determined by three mass measurement techniques and two radiochemical techniques. It is suggested that a conversion factor of 0.79 pCi/μg be used to determine compliance with the California MCL.

Occupational exposure to plutonium is generally monitored through analysis of urine samples. Typically, plutonium is separated from the sample and other actinides, and the concentration is determined using alpha spectroscopy. Current methods for separations and analysis are lengthy and require long count times. A new method for monitoring occupational exposure levels of plutonium has been developed, which requires fewer steps and overall less time than the alpha spectroscopy method. In this method, the urine is acidified, and a Pu internal standard is added. The urine is digested in a microwave oven, and plutonium is separated using an Eichrom TRU Resin column. The plutonium is eluted, and the eluant is injected directly into the Inductively Coupled Plasma-Mass Spectrometer (ICP-MS). Compared to a direct "dilute and shoot" method, a 30-fold improvement in sensitivity is achieved. This method was validated by analyzing several batches of spiked samples. Based on these analyses, a combined standard uncertainty plot, which relates uncertainty to concentration, was produced. The MDA95 was calculated to be 7.0 × 10 μg L, and the Lc95 was calculated to be 3.5 × 10 μg L for this method.