The mechanical properties of biological tissue provide information related to the strength and integrity of the cellular microstructure. Recently, mechanical properties of the brain have been visualized and measured non-invasively with magnetic resonance elastography (MRE). Virtual MRE (VMRE) is based on non-Gaussian distribution model, which can be regarded as an indicator to reflect the complexity and change of different brain microstructure. The purpose of this study is to explore the capability of diffusion–based VMRE in the characterization of the gray matter shear stiffness, discuss the cause of the differences of gray matter encephalic in normal human brains.