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Virus-specific complementary ribonucleic acid (RNA) from cells infected with Sendai virus was isolated by a procedure involving hybridization with virion RNA and isopycnic centrifugation of the RNA hybrids. The complementary RNA contained adenylate-rich sequences which sedimented at about 45.

3'-Deoxyadenosine (75 to 100 jug/ml) permitted analysis of the cytoplasmic influenza virus-specific RNAs synthesized early in the replicative cycle-a phase that has hitherto been obscured by host cell RNA synthesis. In addition, late in the cycle (6 to 8 h) complementary virus-specific RNAs were the predominantly labeled species, suggesting that higher concentrations of 3'-deoxyadenosine selectively inhibit influenza viral genome replication.