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Gelatin/chitosan particles suitable for application in ocular drug administration were prepared by a two-step cross-linking process performed in an emulsion-phase separation system. The particles were characterized by scanning electron microscopy and laser diffractometry, and the diameters were 0.202—4.596 µm. The microparticles pH-dependent behavior was monitored by their mean diameter changes in aqueous environment. Adrenalin was drug used to study loading and release characteristics. The prepared particles were nontoxic, with the DL50 values of 6.9—8.19 g/kg body mass. The in vivo biocompatibility tests consisted of subcutaneous administration of a microparticle suspension in physiological serum followed by morpho histological analysis of the implantation site. The in vivo adrenalin ocular delivery was tested on both animals and a voluntary human patient to determine the adrenalin action and by tears. The particles showed good adherent properties without irritation to the patient; adrenalin was released cleared the ocular congestion.

Oligo(3‐OH butyrate)‐β‐cyclodextrin esters (PHB‐CD) were obtained through ring opening of β‐butyrolactone (β‐BL) in the presence of β‐cyclodextrin (CD) and (‐)‐sparteine (SP) as nucleophilic activator. The resulted reaction mixture was first separated in two fractions and then investigated through a deep mass spectrometry (MS) study performed on a liquid chromatography‐electrospray ionization‐quadrupole time of flight (LC‐ESI‐QTOF) instrument. LC MS and tandem MS structural assignment of the reaction products was completed by NMR. The performed analysis revealed that poly(3‐OH butyrate) homopolymers (PHB) are formed together with the PHB‐CD products. Secondary reactions resulting in the formation of crotonates were also proved to occur. A comparison between MS and NMR results demonstrated that more than one PHB oligomer is attached to the CD in the PHB‐CD product. The tandem MS fragmentation studies validated the proposed structure of CD derivatives. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010