Based on the importance of using low-risk compounds to protect beneficial agents, the present study evaluated the acute toxicity of lethal and sublethal concentrations of the insecticides thiacloprid, pyriproxyfen, and chlorantraniliprole on adults of Aphelinus mali (Haldeman), the main parasitoid of the woolly apple aphid, Eriosoma lanigerum (Hausmann). Similarly, acetamiprid, buprofezin, pyriproxyfen, chlorantraniliprole and cyantraniliprole were evaluated on adults of the parasitoid Acerophagus flavidulus (Brethés) and also on larvae and adults of the predator Cryptolaemus montrouzieri Mulsant, both species important natural enemies of the obscure mealybug, Pseudococcus viburni (Signoret). Natural enemies were exposed to insecticide residues of minimum recommended rate (1x) and between one to four concentrations (0.5x, 0.25x, 0.1x and 0.05x) of the minimum recommended rate on apple leaves. Our results indicate that thiacloprid was moderately harmful to A. mali at 1x, and acetamiprid was harmful to A. flavidulus causing 100% mortality from 0.1x to 1x concentrations. Buprofezin, pyriproxyfen and chlorantraniliprole were harmless or slightly harmful for both parasitoids. Cyantraniliprole was slightly harmful from 0.25x to 1x for A. flavidulus. For C. montrouzieri, acetamiprid was harmless or slightly harmful for larvae and adults on the concentrations evaluated. Buprofezin, pyriproxyfen and chlorantraniliprole were harmless for larvae and adults of C. montrouzieri. Cyantraniliprole was slightly harmful on larvae and moderately harmful on adults of C. montrouzieri at 1x. Our data suggest that buprofezin, pyriproxyfen and chlorantraniliprole could be compatible with the natural enemies evaluated, while thiacloprid and acetamiprid were less compatible with parasitoids than with the predator. Finally, cyantraniliprole seems to be less compatible than chlorantraniliprole at 1x with the natural enemies evaluated.