The act of blowing the whistle on wrongdoing poses an ethical dilemma to the individual, the organization, and society. To help identify the key individual and organizational determinants that encourage or prohibit whistle-blowing in the U.S. federal government, this article presents a logistic regression analysis of survey data collected by the Merit Systems Protection Board, covering 36,926 federal employees from 24 agencies. Findings suggest that, although whistleblowing is a rare event within most federal agencies, its likelihood is positively associated with norm-based and affective work motives, but negatively associated with several key indicators of organizational culture, including perceptions of respect and openness, cooperativeness and flexibility in the work setting, and fair treatment and trust in supervisors. This indicates intrinsic individual motives, together with organizational culture and leadership, should be taken into account when developing and sustaining policies to promote ethical behavior and responsible public service in the federal government. Veterans Affairs. 4. These sections include the following: agency, job, work unit, job performance, pay and rewards, fairness, supervisor, training, career plans, supervisory status, management perspective, employment facts, demographics, and some open-ended questions (mission, performance, employee hiring). Separately, respondents were asked how strongly their perceptions were about these topics. 5. The steps in the exploratory factor analysis comprised running a principal component factoring (pcf) analysis in Stata, analyzing the covariance matrix and factor loadings, rotating the solution (orthogonal rotation using the varimax criterion), and deciding on the number of common factors to be retained based on the eigenvalue criterion (values greater than 1) as well as conceptual considerations. 6. Although the measurement of any phenomenon always contains a certain amount of chance error, this study considered the particular properties of indicators to address the extent to which these are reliable-provide consistent results across repeated measurements. Following Carmines and Zeller (1979), the reliability of the empirical measurements was established before assessing their validity using statistical procedures of internal consistency (Cronbach's alpha for scale construction).