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Introduction:The identification of BCR-ABL expression as the defining leukemogenic event in chronic myeloid leukemia (CML) and the introduction of BCR-ABL tyrosine kinase inhibitors in 2001 have revolutionized disease management, leading to a reduction in mortality rates and accordingly an increase in the estimated prevalence of CML.Case report:Based on medical records and clinical follow-up, the authors present the case of a Philadelphia chromosome–positive CML patient who developed resistance to imatinib. Quantitative reverse transcription-polymerase chain reaction testing revealed a V280G BCR-ABL mutation.Discussion and conclusions:This is the first report describing a new BCR-ABL kinase domain mutation—V280G—that might be associated with resistance to imatinib. Approximately 15% to 30% of patients treated with imatinib discontinue treatment due to resistance or intolerance. More than 90 BCR-ABL mutations were detected so far, conferring variable degrees of drug resistance, with consequent clinical, therapeutic, and prognostic impact.

Primary effusion lymphoma is a rare and aggressive large B-cell lymphoma presenting as malignant effusion with poor prognosis. Although it is more prevalent among HIV patients, it has also been described in non-HIV immunocompromised individuals. Given its rarity, there are no large randomized trials regarding the best therapeutic option. The choice of therapy is based on retrospective studies, case reports and preclinical data. We present the case of a non-HIV patient with relapsed disease after treatment with CHOP who was then successfully treated with brentuximab vedotin, achieving complete remission.

African swine fever virus (ASFV), the causative agent of African swine fever in domestic pigs (3,8,12), is a large icosahedral virus with a double stranded DNA genome of (14) and gene 19R of human herpes virus 6-HH6 (9). Furthermore, each of six highly conserved motifs identified for the helicase superfamily (7) and required for function of the origin binding protein (UL9) of HSV1 are conserved in LMW6DL (10). Sequence analysis with the FASTA algorithm (13) shows LMW6DL most closely related to HH6 (28.5% amino acid identity/341 residues; 51.3% similarity, FASTA = 289, z = 25.2), followed by VZV (25.9% amino acid identity/312 residues; 51.7% similarity, FASTA = 177, z = 15.4), HSV1 (27.5% amino acid identity/325 residues; 50.7% similarity, FASTA = 126, z = 10.6) and EHSV 1 (26.8 % amino acid identity/325 residues; 51.2% similarity, FASTA = 139, z = 1 1.5). The aligment of each of the six highly conserved motifs is shown in Figure 1. UL9 of Herpes simplex virus is essential for viral DNA replication, codes for a protein that specifically recognizes sequences within the viral origin of replication, and possesses independent helicase and DNA-dependent ATPase activities (10). The striking relationship between LMW6DL and the Herpes virus origin binding protein indicates a possible role for this gene product in ASFV DNA replication.

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