As Human Gastric Cancer Death Rates (HGCDR) is unevenly distributed in western Venezuela, we explored possible risk factors, among which Helicobacter pylori (Hp) infection alongside bracken fern invasion could be differential contributors. A prospective transversal study for Hp seroprevalence was conducted in a large number of healthy individuals (15-65 years) in two geographically distinct zones (low megathermic and mountain mesothermic, 0-3000 meters) employing IgG Hp ELISA response at district level. Results were matched with HGCDR records. Hp prevalence was high and statistically similar (p > 0.05) in all populations of both sexes [lowlands: 85.0% (CI 95% 72.7-97.3); highlands: 87.2% (CI 95% 83.2-92.3); p = 0.1138], males more than females in both regions [RR M/F : lowlands 1.17 (CI 95% 0.99-1.36); highlands 1.16 (CI 95% 1.06-1.25)], with the middle aged group being the most affected [lowlands: 94.6% (CI 95% 68.4-119.7); highlands: 86.0% (CI 95% 60.3-111.8)]. A clear HGCDR-elevation gradient was found in both sexes but no association with Hp prevalence. Differential bracken fern invasion in cattle farmland is proposed as a relevant HGCDR risk factor.