SummaryBlood flow is important for the healing of bone fractures. Until now, however, there have been no publications on the daily, continual measurement of intramedullary blood perfusion using laser Doppler flowmetry (LDF) in the conscious animal. In this study, a model for the daily, continual measurement of intramedullary blood perfusion by LDF and the temperature near the cortex both in intact and ostectomized tibiae in the conscious rabbit is described. The probes for blood perfusion and temperature measurement were implanted permanently at three different localizations into the right tibia of 10 adult New Zealand White rabbits. The probes were held in place by a bilateral, single-plane external fixator. In five of these animals, a midshaft tibial ostectomy was created in order to simulate a fracture. Intramedullary blood perfusion and temperature were measured daily over 49 days.While in intact tibiae no significant (P . 0.05) differences were found in blood perfusion readings taken at various time points, for mean values or for blood perfusion over time, in ostectomized tibiae the differences were significant: various time points (P ¼ 0.0056), mean values (P ¼ 0.0034) and blood perfusion over time (P ¼ 0.0337). Blood perfusion readings at the centre probe were elevated compared with those at the proximal and distal probes.Thus, a revascularization in the ostectomy gap during the fracture healing was proven by means of the LDF. No influence of the blood perfusion on the temperature in the ostectomy area could be determined during healing of the ostectomy. The described model seems suitable for the continual measurement of intramedullary blood perfusion both in intact and ostectomized tibiae in the conscious rabbit.