The objective of this review was to evaluate the structural and functional role of Microbial exopolysaccharides (EPS) in food products. EPS are biologically produced natural macromolecules that provides distinctive rheological and physicochemical properties to food matrix. Understanding the interactions of EPS with food components at the molecular level enables the application in bakery products, dairy/non-dairybased functional products, processed meat, as probiotics delivery vehicles, etc. Further information at the biological level throws light on the potential use for immunomodulation, microbiota modulation, and gut-health management. At present, though the commercial applications of these polysaccharides are limited due to lower yield, the bio-thickening properties at lower concentrations increases its scope for structural and functional food production through ex-vivo/in-vivo applications. The information relating to the successful application of EPS for functional foods is scattered and the exploited structural-functional relationships are crucial for laying further investigations to develop futuristic products. This article discusses the application of EPS in functional food products and the prospective benefits.
The main aim of this study was to chemically modify mango kernel starch (MKS) and assess its impact on packaging film properties. MKS exhibited limited application due to low solubility, retrogradation, higher gelatinization temperature and hydrophilicity. These limitations could be effectually overcome by integrating modifications to native starch. Chemical modifications of MKS were performed by oxidation and benzylation with a low degree of substitution. X‐ray diffractogram presented A‐type pattern of crystal structure which was in accordance to endosperm starch. Fourier‐transform infrared (FTIR) spectra of the native and modified starch samples exhibited strong bands in the regions corresponding to OH and CH stretching. Additionally, absorption bands were found in the regions for carbonyl and aromatic phenyl vibrations signifying the substitution of functional groups within native starch. Modified starch films presented increased lightness L* representing that the films were lighter in colour than native film. Tensile strength (TS) of modified films was found to increase more than twice that of native starch film. Starch recrystallization was interrupted by oxidation thereby preventing retrogradation. Benzylation lowered the water vapour permeability by 58% and improved UV absorption by 80% when compared with native film.
Purpose
– The purpose of this paper is to speak about the production of biodiesel from waste cooking oil which serves as an alternate fuel in the absence of conventional fuels such as diesel and petrol. Though much research work was carried out using non-edible crops such as Jatropha and Pongamia, cooking oil utilized in bulk quantity is discarded as a waste. This is reused again as it contains more of esters that when combined with an alcohol in presence of an enzyme as a catalyst yields triglycerides (biodiesel).
Design/methodology/approach
– The lipase producing strain Rhizopus oryzae and pure enzyme lipase is immobilized and treated with waste cooking oil for the production of FAME. Reaction parameters such as temperature, time, oil to acyl acceptor ratio and enzyme concentration were considered for purified lipase and in the case of Rhizopus oryzae, pH, olive oil concentration and rpm were considered for optimization studies. The response generated through each run were evaluated and analyzed through the central composited design of response surface methodology and thus the optimized reaction conditions were determined.
Findings
– A high conversion (94.01 percent) was obtained for methanol when compared to methyl acetate (91.11 percent) and ethyl acetate (90.06 percent) through lipase catalyzed reaction at oil to solvent ratio of 1:3, enzyme concentration of 10 percent at 30°C after 24 h. Similarly, for methanol a high conversion (83.76 percent) was obtained at an optimum pH of 5.5, olive oil concentration 25 g/L and 150 rpm using Rhizopus oryzae when compared to methyl acetate (81.09 percent) and ethyl acetate (80.49 percent).
Originality/value
– This research work implies that the acyl acceptors methyl acetate and ethyl acetate which are novel solvents for biodiesel production can also be used to obtain high yields as compared with methanol under optimized conditions.
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