Honey produced by stingless bee of Trigona spp. is popularly known as Kelulut honey (KH) in Malaysia. Even though KH has been increasingly accepted by Malaysians, information relating to its physical and antioxidant properties is still limited. This study aimed to determine the physical properties, antioxidant content and anti-oxidative properties of KH collected from different regions in Peninsular Malaysia. Physical properties of KH including total soluble solids, pH, moisture, ash content, and colour were determined. Antioxidant content namely total phenolics, flavonoids, carotenoids, ascorbic acid equivalent antioxidant content (AEAC) and quercetin equivalent antioxidant content (QEAC) were also quantified. Anti-oxidative potential of KH was assayed using DPPH radical scavenging and FRAP assays. KH has pH of 3.29-3.71, total soluble solids (66.23-73.70 °Brix), 21.40-31.59% moisture and 0.22-0.41% ash content. Colour measurement showed KH from the northern region was lightest (L = 39.32) while KH from east coast (b = -5.06) and central (a = 5.65) regions were more pronounce in blue and red colours. KH from east coast region showed highest values for phenolics (1169.36±51.11 mg GAE/kg), flavonoids (79.13±0.49 mg QE/kg), carotenoids (4.61±0.38 mg/kg), AEAC (146.20±5.56 mg/kg) and QEAC (177.08±5.54 mg/kg). In line with the antioxidant content, honey from the east coast region also had strongest anti-oxidative activities indicated in its lowest IC 50 value of DPPH radicals (15.07±1.05 mg/mL) and highest FRAP value (7477.03±48.80 μMFe(II)/kg). The KH collected from different regions showed varied physical and antioxidant attributes which may due to variety source of floral origin.

The notion of fruit and vegetables (FV) in preventing chronic diseases has long been discerned. To meet the recommended FV intake, FV juices have emerged as a convenient and healthy choice. Mixed fruit and vegetable juices (MFVJ) cater the blend of desirable flavor and taste of consumers. This study was carried out to determine the total phenolic content (TPC), antioxidant activities and sugar content of selected MFVJ. Folin-Ciocalteu assay was employed to quantify TPC, whereas DPPH and FRAP assays were used to evaluate the antioxidant activities of MFVJ. The sugar content was determined using phenol-sulfuric acid method. MFVJ extracted from bitter gourd, green apple and orange (BGO) had the highest TPC content (76.4 ± 4.2 mg GAE/100 ml). Celery and green apple (CG) juice showed the highest DPPH value (522.3 ± 7.6 mg TE/100 ml) whereas carrot and starfruit (AS) juice have the highest FRAP value (419.6 ± 21.6 mg TE/100 ml). The sugar content of MFVJ was within the range of 5.7-13.3 g/100 ml. MFVJ can be considered as healthy beverages with considerable amounts of phenolic compounds and low sugar content. This study provides some useful reference for consumers who consume juices with combinations of FV. Future studies need to discover more combinations of FV juices, providing more data pertaining to MFVJ. Identification of individual phenolic compounds should also be part of future research using various instrumental analyses.