No abstract

In this present study, eight genotypes of Brassica juncea comprising four tolerant (IC-399802, IC-491089, IC-312545, IC-312553) and four susceptible (IC-385686, IC-264131, IC-426392, Laxmi) genotypes for aphid tolerance were used to generate Sequence Characterized Amplified Region (SCAR) markers through dominant PCR based markers (51 RAPD and 12 ISSR markers). 13 RAPD (Random Amplified Polymorphic DNA) and 8 ISSR (Inter Simple Sequence Repeat) markers were found to be polymorphic but only 3 primers OPE16 (RAPD), UBC 839 (ISSR) and UBC 864 (ISSR) were obtained which could distinguish tolerant genotypes from susceptible ones. OPE16 (RAPD) obtained unique band of size approx. 600 bp in susceptible genotypes while it was absent in the tolerant genotypes. Similarly, UBC 839 (ISSR) yielded ~800 bp unique band in bulk tolerant while UBC 864 (ISSR) yielded three bands of ~1200 bp, ~1000 bp, and ~500 bp in tolerant genotypes which was absent in the susceptible genotypes. These unique bands were excised to generate five sets of SCAR markers. Among the five sets of SCAR marker, only BJSCAR F1 and BJSCAR R1 set yielded the promising result in all for 4 susceptible genotypes as well as bulk susceptible and was absent in all the tolerant genotypes and Brassica fruticulosa (highly tolerant to aphid, used as a control). So, this SCAR marker developed could be successfully used in screening of B.juncea genotypes in future breeding programs.

were crossed in a diallel (half) pattern to get 21 hybrid to investigate extent of heterosis fruit yield and quality traits in bitter gourd at vegetable research farm, Bihar agricultural university, Bihar during summer season 2016 and 2017. The cross combination Pusa Ausadhi X BRBT Local exhibited maximum heterosis over standard check for yield and yield attributing trait like number of primary branch, vine length, fruit length and fruit weight. In case of quality parameter, BRBT Local X Konkan Tara exhibit highest magnitude of heterosis for ascorbic acid and chlorophyll content. K e y w o r d sBitter gourd and Heterosis

The present investigation was carried out on thirty-six lentil genotypes at the farm of Bihar Agricultural University, Sabour, Bhagalpur in 2018-19. The genetic divergences were examined using Mahalanobis D2 statistics for two dates of sowing i.e normal sown and late sown condition. The presence of variation among genotypes was revealed by ANOVA for considerable traits which come out as significant for all traits under study except several primary branches per plant, the number of pods per cluster under both normal and late sown condition and the number of seeds per pod in late sown condition. The study on genotypic variability, correlation and path analysis was done to know the direct and indirect relationship on seed yield and yield components for enhancement of economic yield. The total genotypes were grouped into 7 clusters under a timely sown condition such as. Cluster I being the largest comprising 10 genotypes, followed by cluster II with 8 genotypes, cluster III with 7 genotypes, cluster IV had 6 genotypes. Cluster V with 3 genotypes and cluster VI and cluster VII was monogenic. Coming to late sown condition, five clusters were formed displaying cluster II with a maximum number of genotypes (18 genotypes) followed by cluster I (11genotypes), cluster III (5 genotypes) and lastly, cluster IV and V were Adan et al.; CJAST, 39(2): 57-62, 2020; Article no.CJAST.50168 58 monogenic. 100 seed weight contributed maximum towards genetic divergence followed by grain yield per plant and number of pods per plant in timely sown and 100 seed weight chased by the number of pods per plant and number of secondary branches per plant under a late sown environment which can be integrated into the enhancement of productivity of lentil. Owing to the results of inter-cluster distance and mean performance of clusters for different traits, the genotypes such as FLIP 2010-86L and X2011S-172-1 under normal sown and likewise, Pusa Vaibhav and IPL-406 under late sown environment are expected to trigger the heterotic combination and obtain the desirable segregants for yield and its determinants. Original Research Article

Rapeseed-mustard has significant role to play to meet the growing demand of edible oils from domestic production. The productivity of this crop is greatly affected by much biotic and abiotic stress. Among biotic stresses