The plant selected in the present study for analysing the antibacterial activity is, Alambusha (Biophytum sensitivum). The whole part of the plant is considered useful and exhibits krimighna, vrana ropana and pitta kaphaghna action as per Ayurveda. Also, it has been said by the folklore people that the leaves exhibit styptic action. So combining all these views, the study goes step by step to compare the Antibacterial activity of the leaf, root and leaf root combination of Alambusha in three different extraction solvents (Ethanol, chloroform, aqueous), which is taken at a fixed concentration of 20 mg/ml (2%) in an increasing volume of 25 ul, 50 ul, 75 ul, 100 ul against two wound infection-causing bacteria’s such as Pseudomonas aeruginosa andBacillus subtilis, by Agar well diffusion method. The study fortifies that leaf ethanolic extract of Alambusha at a volume of 100 microliters shows maximum antibacterial activity against both bacterial species, that too highest in Pseudomonas aeruginosa when compared to root alone and leaf along with root combinations. These results obtained show that plant extracts can be used as anti-infective agents.