BackgroundThe molecular mechanisms underlying the development and progression of gastric carcinoma remain poorly understood. The main objective of this study was to investigate the expression level of targeting protein for Xenopus kinesin-like protein 2 (TPX2) and its clinical significance in human gastric carcinoma.MethodsReal-time quantitative polymerase chain reaction (RT-PCR) and western blotting were used to determine the mRNA and protein levels of TPX2 in 20 paired gastric carcinoma tissues and the adjacent normal tissues, and the expression of TPX2 protein in 106 specimens of a gastric carcinoma tissue microarray was determined by immunohistochemistry. The associations of TPX2 expression with the clinicopathological features were analyzed, and the prognosis of gastric carcinoma patients was evaluated.ResultsThe results showed that the expression of TPX2 mRNA was significantly higher in gastric carcinoma than in the adjacent normal tissues in 20 paired samples. Western blotting analysis revealed that TPX2 protein was differentially increased in 17 of 20 specimens from primary human gastric carcinoma tissues compared with those from adjacent non-tumor tissues. Immunohistochemical staining showed that TPX2 over-expression was significantly associated with advanced age (P = 0.001) and tumor T stage (P = 0.003). In addition, TPX2 was an independent prognostic factor for overall survival (OS) in the multivariate analysis [hazard ratio (HR) 0.001; 95 % confidence interval (CI) 2.626–7.198; P = 0.001].ConclusionsTPX2 is up-regulated in gastric carcinoma and is associated with old age and tumor T stage. TPX2 may serve as a good prognostic indicator in patients with gastric carcinoma.Electronic supplementary materialThe online version of this article (doi:10.1186/s12935-016-0357-7) contains supplementary material, which is available to authorized users.

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Magnetotelluric (MT) impedance and tipper data can be utilized to reflect underground structural information. However, when the axial anisotropic property of an abnormal body is marked, it will seriously influence MT measuring data. This study divides the MT field into primary and secondary components and replaces the conductivity parameter in a three-dimensional (3D) governing equation with axial anisotropic conductivity. Analysis of the influence of axial anisotropy on MT tipper data is presented, and a limited-memory Broyden-Fletcher-Goldfarb-Shanno method is then utilized to realize a 3D MT axial anisotropic inversion with impedance and tipper data. The tests presented in this paper show that the resolution of the inverting axial anisotropic model with impedance and tipper data is better than that of only using impedance data.