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Chrysophanol is an anthraquinone compound, mainly isolated from rhubarb, with anti-cancer effects on some types of cancer cells. However, effects of chrysophanol on human choriocarcinoma cells are not known. Therefore, the objective of this study was to determine effects of chrysophanol on choriocarcinoma cells (JAR and JEG-3) and identify signal transduction cascades activated by chrysophanol. Results of present study, showed that chrysophanol decreased cell viability and induced apoptosis of JEG-3, but not JAR cells, in a dose-dependent manner. Chrysophanol also increased oxidative stress in JEG-3 cells by inducing ROS generation followed by mitochondrial dysfunction including depolarization of mitochondrial inner membrane potential. Western blot analysis revealed that ERK1/2, P90RSK, AKT, and P70S6K were increased significantly in JEG-3 cells by chrysophanol. Next, we investigated chrysophanol-mediated effects on proliferation of JEG-3 cells using pharmacological inhibitors of PI3K/AKT (LY294002) and ERK1/2 (U0126). Inhibition of AKT and ERK1/2 prevented chrysophanol-induced stimulation of proliferation of JEG-3 cells. In addition, the phosphorylation of AKT and ERK1/2 was suppressed by LY294002 and U0126 in JEG-3 cells treated with chrysophanol, whereas, the AKT protein was activated by pre-treatment of JEG-3 cells with U0126. Furthermore, we compared therapeutic effects of chrysophanol with cisplatin and paclitaxel which are conventional salvage regimens for choriocarcinoma. Our results verified that chrysophanol has synergistic effects with traditional therapy to increase apoptosis of JEG-3 cells. Collectively, these results indicate that chrysophanol is a potential effective chometherapeutic agent for treatment of choriocarcinoma therapy, and minimizing side effects of conventional treatment regimens. J. Cell. Physiol. 232: 331-339, 2017. © 2016 Wiley Periodicals, Inc.

Axial cooling air ducts in the rotor of large induction motors are known to produce magnetic asymmetry, and can cause steady state current or vibration spectrum analysis based fault detection techniques to fail. If the number of axial air ducts and poles are identical, frequency components that overlap with that of rotor faults can be produced for healthy motors. False positive rotor fault indication due to axial ducts is a common problem in the field that results in unnecessary maintenance cost. However, there is currently no known test method available for distinguishing rotor faults and false indications due to axial ducts other than off-line rotor inspection or testing. Considering that there is no magnetic asymmetry under high slip conditions due to limited flux penetration into the rotor yoke, detection of broken bars under the startup transient is investigated in this paper. A wavelet-based detection method is proposed and verified on custom-built lab motors and 6.6 kV motors misdiagnosed with broken bars via steady state spectrum analysis. It is shown that the proposed method provides reliable detection of broken bars under the startup transient independent of axial duct influence.

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