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Several brands of microaggregate blood filters were evaluated in vitro and in vivo for their effect on plasma free hemoglobin and platelet count, both while new and after prior usage.Time required for passage of diluted packed red cells was also determined. The authors conclude that, although the use of microaggregate filters for multiple transfusions is commonly accepted, their use with fresh blood may decrease platelet count. Filtration characteristics and administration time should be considered in selection of filters.