The objective of this work was to screen molds for antioxidant activity. The results of screening 10 molds showed that three, Aspergillus candidus CCRC 31543, Aspergillus sp. CCRC 31742, and Penicillium oxalicum CCRC 31670, produce antioxidants that can be extracted from broth culture filtrates by ethyl acetate. The most antioxidant activity was found in the ethyl acetate extracts of A. candidus CCRC 31543. The antioxidant activity of A. candidus CCRC 31543 was as strong as that of rosemary extracts and butylated hydroxyanisole and was greater than that of α-tocopherol at the same concentrations. No antioxidant activity was found in aqueous extracts of A. candidus broth filtrate. A. candidus was cultured in a 500-ml Hiton flask containing 100 ml of growth broth, which consisted of 3% sucrose, 0.1 % yeast extract, 0.1 % polypeptone, 0.3% NaNO3, 0.1% K2HPO4, 0.05% MgSO4·· 7H2O, 0.05% KCl, and 0.001 % FeSO4 · 7H2O, at an incubation temperature of 25°C for 15 days, and a shaking rate of 130 rpm. The antioxidant activity of A. candidus reached a maximum level after 13 days of culture.
In this paper, three asynchronous FIFO design styles, linear, square, and cubic, are investigated based on the Balsa synthesis system. These styles are designed with the key difference being the path by which data travels through the FIFO. The design with shorter path should result in lower latency and higher throughput, but will require more complicated control. All the FIFOs are designed using the Balsa language, and the area cost and simulation time are compared for each FIFO with varying sizes. A tool is also presented for automatic generation of Balsa code for each FIFO.
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