Although constructed wetlands (CWs) are widely used around the world with various substrates, the mechanisms of how these modified substrates affect wastewater treatment are still unknown. In this study, CW microcosms were established with and without ceramsite as a substrate, and the wastewater treatment efficiencies were evaluated during 71 days of incubation. Using the 16S rRNA high-through sequencing, the mechanisms of how CW substrate changed the microbial community was quantified. The results showed that compared to soil as substrate, the use of ceramsite as substrate material enhanced the removal of pollutants from CW systems, particularly under a short retention time (1.5-day) condition. There were more beneficial microorganism groups (nitrogen, sulfur, phosphate) in the ceramsite CW system than the non-ceramsite CW system, particularly in the bottom layers. Moreover, the CW with ceramsite substrate had more nitrification function. All of these results suggested that the ceramsite CW system enhanced the removal of pollutants because it increased the concentration of key microbes that are necessarily for nutrient cycles.

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Discharge of wastewater contained high amount of nitrogen would cause eutrophication to water bodies. Simultaneous nitrification and denitrification (SND) has been confirmed as an effective process, the isolation of SND bacteria is crucial for its successful operation. In this study, an SND strain was isolated and identified as Pseudomona aeruginosa LS82, which exhibited a rapid growth rate (0.385 h−1) and good nitrogen removal performance (4.96 mg N·L−1·h−1). Response surface methodology was applied to optimize the TN removal conditions, at which nearly complete nitrogen (99.8 ± 0.9%) removal were obtained within 18 h at the condition: pH 8.47, 100 rpm and the C/N ratio of 19.7. The saddle-shaped contours confirmed that the interaction of pH and inoculum size would influence the removal of total nitrogen significantly. Kinetic analyses indicated that the reduction of nitrite was the rate-limiting step in the SND process. Our research suggested strain LS82 can serve as a promising candidate for the treatment of ammonium rich wastewater, and expended our understanding the nitrogen removal mechanism in the SND process.