The present study was aimed to evaluate the improvement potential of tea polyphenols (TPP) on silage characteristics and bacterial community. Stylo ensiled with TPP (0, 0.2 or 0.4%, on a fresh basis) were analyzed for fermentation parameter, protein fraction, antioxidant activity and bacterial community after 7, 14, 30 and 60 days fermentation. The addition of TPP resulted in the decrease (P < 0.05) of pH values (5.09 vs. 4.91), dry matter loss (11.77 vs. 8.02% DM), butyric acid concentration (1.64 vs. 1.02% DM) and ammonia-N proportion (13.69 vs. 8.98% CP, on Day 30) of stylo silage as well as the increase (P < 0.01) of lactic acid bacteria population (6.17 vs. 7.54 cfu/g FM) and true protein content (6.03 vs. 7.44% DM), particularly at the first 30 days of ensiling. It somewhat enhanced the antioxidant capacity of style silage at the early stage, and altered the bacterial community of stylo silage, with Clostridium_sensu_stricto_1 and Lachnoclostridium_5 much decreased but Enterobacter and Clostridium still being the dominant genera. It is suggested that TPP could help improve fermentation quality and nutrient preservation of stylo silage, and delay proteolysis process and antioxidant decay.
Use of driver seat belts and availability and functionality of passenger seat belts in a convenience sample of 231 Beijing taxis were examined in the months prior to the 2008 Beijing Olympic Games. Driver and front passenger seat belt use was mandated in China from 2004 to help address the growing public health crisis of road trauma. Results from observations made by in-vehicle passengers revealed that 21.2% of drivers were correctly wearing a belt, approximately half were not, and one third were using the belt in a non-functional way. Over 3/4 of this sample of taxi drivers were unrestrained while working. The percentage of functionally available belts was higher for front than rear passengers (88.3% and 22.9%, respectively). This low rate of belt availability in rear seats calls into question the preparedness of the fleet to cater for the safety needs of foreign visitors to China, particularly those from countries with high levels of restraint use. Factors influencing the use/misuse of seat belts in China remain largely unexplored. Results of this pilot study support further investigations of barriers to using injury prevention mechanisms such as seat belts in less motorised countries.
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