PurposePrevious studies have confirmed that microRNAs play important roles in the pathogenesis of acute aortic dissection (AAD). Here, we aimed to explore the role of miR-145 and its regulatory mechanism in the pathogenesis of AAD.Materials and MethodsAAD tissue samples were harvested from patients with aortic dissection and normal donors. Rat aortic vascular smooth muscle cells (VSMCs) were transfected with miR-145 mimic/inhibitor or negative control mimic/inhibitor. Gene and protein expression was measured in human aortic dissection tissue specimens and VSMCs by qRT-PCR and Western blot. Luciferase reporter assay was applied to verify whether connective tissue growth factor (CTGF) was a direct target of miR-145 in VSMCs. Methyl thiazolyl tetrazolium assay was used to detect VSMC viability.ResultsmiR-145 expression was downregulated in aortic dissection tissues and was associated with the survival of patients with AAD. Overexpression of miR-145 promoted VSMC proliferation and inhibited cell apoptosis. Moreover, CTGF, which was increased in aortic dissection tissues, was decreased by miR-145 mimic and increased by miR-145 inhibitor. Furthermore, CTGF was confirmed as a target of miR-145 and could reverse the promotion effect of miR-145 on the progression of AAD.ConclusionmiR-145 suppressed the progression of AAD by targeting CTGF, suggesting that a miR-145/CTGF axis may provide a potential therapeutic target for AAD.

Volume ignition is a method of igniting a fuel as a whole by simultaneously achieving ignition conditions throughout the fuel zone. The basic criterion for ignition is that the thermonuclear energy is greater than the energy leakage at the fuel boundary, resulting in self-sustaining heating and deep combustion. Deuterium-tritium fuels are wrapped in medium to high Z media to reduce radiative leakage and achieve lower-temperature holistic ignition and non-equilibrium combustion, ultimately allowing the fuel to achieve high combustion efficiency. Volume ignition is the use of energy balance relations under the local thermodynamic equilibrium approximation to establish the energy balance equation for thermonuclear systems, and the system ignition threshold is obtained by solving this equation. By understanding the physical process, we believe that the non-equilibrium process is universal to the volume ignition process. Changes in external factors (density, boundaries, albedo, etc.) at the moment of ignition can have a significant impact on the development direction of the system, and an ignition system with a large surface density can nevertheless withstand a large amount of reverse work and continue to burn. The design of the ignition target tries to avoid these factors through margin design, but conversely, the rational use of these laws can further improve the design margin of the capsule. With the aid of big data, the volume ignition method is easier to calculate and has a shorter iteration time. The traditional way is to propose a model, set the material, and then perform the calculation while using big data can set any model and material for calculation. In this paper, a simple comparison will be made to find out that the efficiency of the physical design of a volume ignition target will be effectively improved with the aid of big data. Volume ignition targets can be used not only in Z-pinch-driven systems but also for laser-driven volume ignition.

In order to investigate the effects of high voltage atmospheric cold plasma (HVACP) treatment on the number of microorganisms in and the quality of Trachinotus ovatus during refrigerator storage, fresh fish was packaged with gases CO2:O2:N2 (80%:10%:10%) and treated by HVACP at 75 kV for 3 min; then, the samples were stored at 4 ± 1 °C for nine days. The microbial numbers, water content, color value, texture, pH value, thiobarbituric acid reactive substance (TBARS), and total volatile base nitrogen (TVB-N) values of the fish were analyzed during storage. The results showed the growth of the total viable bacteria (TVB), psychrophilic bacteria, Pseudomonas spp., H2S-producing bacteria, yeast, and lactic acid bacteria in the treated samples was limited, and they were 1.11, 1.01, 1.04, 1.13, 0.77, and 0.80 log CFU/g−1 lower than those in the control group after nine days of storage, respectively. The hardness, springiness, and chewiness of the treated fish decreased slowly as the storage time extended, and no significant changes in either pH or water content were found. The lightness (L*) value increased and the yellowness (b*) value decreased after treatment, while no changes in the redness (a*) value were found. The TBARS and TVB-N of the treated samples increased to 0.79 mg/kg and 21.99 mg/100 g, respectively, after nine days of refrigerator storage. In conclusion, HVACP can limit the growth of the main microorganisms in fish samples effectively during nine days of refrigerator storage with no significant negative impact on their quality. Therefore, HVACP is a useful nonthermal technology to extend the refrigerator shelf-life of Trachinotus ovatus.