The leaflet vibration phenomenon in bileaflet mechanical heart valves (BMHVs) can cause complications such as hemolysis, leaflet damage, and valve fracture. One of the main reasons for leaflet vibration is the unsteady blood flow pressure pulsation induced by turbulent flow instabilities. In this study, we performed numerical simulations of unsteady flow through a BMHV and observed pressure pulsation characteristics under different flow rates and leaflet fully opening angle conditions. The pressure pulsation coefficient and the low-Reynolds k-ω model in CFD (Computational Fluid Dynamics) software were employed to solve these problems. Results showed that the level of pressure pulsation was highly influenced by velocity distribution, and that the higher coefficient of pressure pulsation was associated with the lower flow velocity along the main flow direction. The influence of pressure pulsation near the trailing edges was much larger than the data obtained near the leading edges of the leaflets. In addition, considering the level of pressure pulsation and the flow uniformity, the recommended setting of leaflet fully opening angle was about 80°.

Understanding the dynamics of biofilm development in response to chemical cues and signals is required toward the development of controllable biofilm-mediated bioprocesses. In this study, we report a new biofilm growth system that integrates a microfluidic gradient mixer with a biofilm growth chamber. The biofilm growth system allows biofilms to grow under defined solute gradients and enables nondestructive monitoring of the biofilm development dynamics in response to the defined gradients.The solute gradients generated in the system were simulated and then validated experimentally. We then demonstrated the applicability of the biofilm growth system in studying biofilm development under defined solute gradients. Specifically, we examined biofilm development of Shewanella oneidensis and Comamonas testosteroni under a defined calcium and nitrate gradient, respectively. Using two C. testosteroni strains (WDL7 and I2), we further demonstrated the applicability of our biofilm growth system to study the development of coculture biofilms under a defined solute gradient. Our results show that the biofilm growth system we have developed here can be a promising tool to reveal the dynamics of biofilm development in response to chemical cues and signals as well as the interorganism interactions in coculture biofilms.