Mungbean (Vigna radiata [L.]) is an important economic crop grown in South, and East Asia. The low contiguity of the current assembly of V. radiata genome has limited its application. Here, we report a high-quality chromosome-scale assembled genome of V. radiata to facilitate the investigation of its genome characteristics and evolution. By combination of Nanopore long reads, Illumina short reads and Hi-C data, we generated a high-quality genome assembly of V. radiata, with 473.67 megabases assembled into 11 chromosomes with contig N50 and scaffold N50 of 11.3 and 42.4 megabases, respectively. A total of 52.8% of the genome was annotated as repetitive sequences, among which LTRs (long terminal repeats) were predominant (33.9%). The genome of V. radiata was predicted to contain 33,924 genes, 32,470 (95.7%) of which could be functionally annotated. Evolutionary analysis revealed an estimated divergence time of V. radiata from its close relative V. angularis of ~11.66 million years ago. In addition, 277 V. radiata specific gene families, 18 positively selected genes were detected and functionally annotated. This high-quality mungbean genome will provide valuable resources for further genetic analysis and crop improvement of mungbean and other legume species.

Seed coat color and anthocyanidin coloration are important traits for variety identification and marker assisted selection in mungbean. Based on the phenotypes of progenies from different combinations, we analyzed the inheritance of the traits above. The results showed that seed coat color was controlled by mono-gene, and green was dominant to yellow, while black was dominant to green. The seed luster had a complicated genetic model, and there was no relationship between seed coat color and luster. The inheritance of anthocyanidin coloration on different parts of plant was also agreeable to mono-gene. However, the relationships among anthocyanidin coloration on different parts of plant were not similar. The genes controlling anthocyanidin coloration in seedlings and in leaf base might be tightly linked together or be the same, while the gene controlling anthocyanidin coloration in keel was not linked with other genes.

No abstract

No abstract