The predominant determinants of α-thalassemia are deletions in the human α-globin gene cluster. A rapid DNA-based assay is needed for mass screening in thalassemia-prevention programs. Herein, we established a novel quadruplex TaqMan qPCR gene dosage assay with two separate combination reactions. The assay directly determined the copy number of human α-globin genes based on relative quantitation of three target genes (HBA2, HBA1, and HBZ or HBPA1) versus a control gene (CREBBP). The assay showed good accuracy, with mean intra-assay and interassay variations of 3.31% ± 1.02% and 5.49% ± 0.32%, respectively. The assay was evaluated using 678 pretyped clinical DNA samples containing six α-thalassemia deletions in 13 genotypes and 186 normal samples previously screened by multiplex ligation-dependent probe amplification or gap PCR. As determined by the 2(-ΔΔCq) method, deleted gene dosage ratios were 0.46 to 0.60 in heterozygotes, 0.0 in homozygotes, and 0.97 to 1.07 in nondeleted samples. We found 99.3% concordance between the quantitative PCR and multiplex ligation-dependent probe amplification or gap-PCR results. Furthermore, routine screening for α-thalassemia deletions was performed on 3000 random samples in a blind analysis. Results for all 279 positives, which had different deletions, were fully coincident with results from standard methods. We also identified two novel deletions confirmed by multiplex ligation-dependent probe amplification. Assays using the novel method are simple and suitable for rapid genotyping and mass screening.

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The digital civilization of human-computer coexistence has become a reality, thanks to the intelligent era. The widespread application of artificial intelligence technology in education has paved a new road for the collaborative and innovative development of ideological and political science teachers in universities and schools. In response to the problems of irregular, unprofessional, and unified resource sharing channels, duplicated, inverted, and disconnected teaching contents, and uncoordinated, insufficient, and unbalanced scientific research linkages in the integration process of university and college civics courses, the B/S three-layer architecture framework is used to build a university and college civics course teachers’ collaborative innovation platform that supports both PC and cell phone use by using jQuery, my structured query language (MySQL), hypertext preprocessor (PHP), juvenile sex offender assessment protocol (J-SOAP) crawlers, and other technologies. Teachers of civics courses can use the platform as a collaborative innovation tool. The goal of integrating resources, teaching, and scientific research of university and college civics courses is achieved, and the collaborative innovation development of university and college Civics teachers is promoted from theory to practice, thanks to the development of three major functions of the platform, namely, resource sharing, scientific research exchange, and teaching mutual assistance.

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In order to direct organizations to implement customer satisfaction improvement effectively, and to help enterprises out of the "customer satisfaction cycle" and " customer satisfaction trap", and then improve the relations with customers continuously, this paper defines the common misunderstandings of customer satisfaction improvement and its reasons, and designs the method of priority improvement rules, measuring and evaluating customer satisfaction index based on the customer satisfaction matrix graph. Trough defining the objects of improvement and its improvement extent with the customer satisfaction matrix graph and customer satisfaction matrix, the prior improvement route can be configured.