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In this study, the in vitro digestibility of corn starch with different amylose content was determined. The results showed that waxy corn starch (WCS) and corn starch (CS) have the highest digestibility, while high amylose corn starch (HACS) has a higher content of resistant starch (RS). Besides being related to amylose content, RS content is also closely related to particle shape, debranched fine structure, molecular structure, and semi-crystalline structure. HACS can maintain a complete particle structure after gelatinization and enzymolysis; differential scanning calorimetry showed a positive correlation between gelatinization enthalpy and RS content. As the amylose content increased, the content of fa (DP 6–12) decreased, while the content of fb2 (DP 25–36) and fb3 (DP ≥ 37) increased, which in-turn decreased the cluster polymer formed by short branch chains, and the formation of more hydrogen bonds between long chain branches improved starch stability. D, which characterizes the compactness of starch semi-crystalline structure, increased with the increase of RS content. HACS 60 with the highest RS content had a unique surface fractal structure between 7.41 < d (2π/q) < 10.58 nm, indicating that the dense structure is effective in maintaining the RS content.

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Gold loaded TiO2 nanoparticles have been synthesized and characterized by powder XRD, HR-TEM, and EDX analysis. The binding interaction of fluorescent sensor 5-amino-2-mercaptobenzimidazole (fluorochrome) with TiO2 and gold loaded TiO2 nanoparticles has been discussed herein. The interaction of fluorochrome with TiO2 and gold loaded TiO2 nanoparticles has been studied by UV-visible, fluorescence, and FT-IR spectral techniques. The fluorescence emission occurs at 421 nm and this has been selectively enhanced by TiO2 nano semiconductor. This technique is sensitive to detect and estimate TiO2 nano semiconductor at a micromolar level. This switch-on fluorescence is suppressed when it is loaded with gold. The strong adsorption of fluorochrome over the surface of nano semiconductor results in the electron transfer between fluorochrome and nano semiconductor. Further, the binding site of nano semiconductor with fluorochrome has been studied theoretically by using the molecular electrostatic potential (MEP). The results show higher electron density at the azomethine nitrogen atom.