Hiking is a popular recreational activity and to cater to public demand, it is apt to increase the number of hiking trails. Various methodologies have been proposed to evaluate the suitability of forest trails to be constructed as hiking trails, but they can be costly and require relevant knowledge in analyzing digital information through a high-throughput dataset. Therefore, there is a need to come up with a simple method to obtain first-hand information on the trail condition, particularly considering the aspects of safety and suitability to hikers, using both on-ground and aerial observations. In this study, we introduce a new assessment approach to analyze and select old forest trails to be reconstructed as new hiking trails. This is useful for park managers who prioritize safety, comfort, and aesthetic features of the recreation site for their visitors. Trail condition assessment was carried out along the trail whereby a 2×2 m sampling plot was constructed at every 100 m. Aerial drone survey was conducted to produce an ortho-mosaic that revealed the percentage of exposed trail from above. Potential phytotourism products and scenic spots were identified and recorded for their locations along the trail to promote the aesthetic value of the recreation site. A strength distribution plot was prepared based on the trail condition, canopy coverage, and aesthetic features along the trail that were categorized using three altitude ranges (n ≤ 150 m, 150 < n < 250 m, n ≥ 250 m a.s.l.). This is to assess the trade-offs in safety, comfort, and aesthetic features along the trail. The development of this methodology offers a direct and cost-effective, yet informative approach to evaluate the quality of a potential hiking trail, thus could effectively aid in the promotion of nature-based tourism.

Pinaceae is the largest family of conifers, dominating forest ecosystems and serving as the backbone of northern, temperate and mountain forests. The terpenoid metabolism of conifers is responsive to pests, diseases, and environmental stress. Determining the phylogeny and evolution of terpene synthase genes in Pinaceae may shed light on early adaptive evolution. We used different inference methods and datasets to reconstruct the Pinaceae phylogeny based on our assembled transcriptomes. We identified the final species tree of Pinaceae by comparing and summarizing different phylogenetic trees. The genes encoding terpene synthase (TPS) and cytochrome P450 proteins in Pinaceae showed a trend of expansion compared with those in Cycas. Gene family analysis revealed that the number of TPS genes decreased while the number of P450 genes increased in loblolly pine. Expression profiles showed that TPSs and P450s were mainly expressed in leaf buds and needles, which may be the result of long-term evolution to protect these two vulnerable tissues. Our research provides insights into the phylogeny and evolution of terpene synthase genes in Pinaceae and offers some useful references for the investigation of terpenoids in conifers.

Our previous study discovered that two urban pest ants, red imported fire ants, Solenopsis invicta Buren (Formicidae: Myrmicinae), and ghost ants, Tapinoma melanocephalum (Fabricius) (Formicidae: Dolichoderinae), can pave viscose surfaces with particles to facilitate food search and transport. We hypothesize that this paving behavior can be applied to monitor S. invicta and T. melanocephalum. In the present study, 3998 adhesive tapes, each with a food source (sausage), were placed in 20 locations around Guangzhou, China (181–224 tapes per location), and their efficiency to detect S. invicta and T. melanocephalum was compared with two traditional ant-monitoring methods, baiting and pitfall trapping. Overall, S. invicta was detected by 45.6% and 46.4% of baits and adhesive tapes, respectively. In each location, the percentage of adhesive tapes detecting S. invicta and T. melanocephalum was similar when compared to baits and pitfall traps. However, significantly more non-target ant species showed up on bait and pitfall traps. Seven non-target ant species—Pheidole parva Mayr (Formicidae: Myrmicinae), Pheidole nodus Smith (Formicidae: Myrmicinae), Pheidole sinica Wu & Wang (Formicidae: Myrmicinae), Pheidole yeensis Forel (Formicidae: Myrmicinae), Carebara affinis (Jerdon) (Formicidae: Myrmicinae), Camponotus nicobarensis Mayr (Formicidae: Formicinae), and Odontoponera transversa (Smith) (Formicidae: Ponerinae)—also showed tape paving behavior, but they can be easily distinguished morphologically from S. invicta and T. melanocephalum. Our study showed that the paving behavior occurs in different subfamilies of ants (i.e., myrmicinae, dolichoderinae, formicinae, and ponerinae). In addition, paving behavior can potentially be used to develop more specific monitoring methods for S. invicta and T. melanocephalum in urban areas in southern China.

Our previous studies have shown that some Trichoderma fungi trigger aggregation behavior in Formosan subterranean termites, Coptotermes formosanus Shiraki. However, the mechanisms underlying the induction of termite aggregation by Trichoderma fungi remain unclear. Here, we found that the aqueous or acetone extract of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg and Trichoderma virens Pers. ex Fries isolated from the gut or cuticle of C. formosanus elicited significant termite aggregation in 2‐choice tests. We then screened 9 Trichoderma metabolites (3‐acetoxy‐2‐butanone, phenol, 3‐ethoxypropionic acid, ethyl 2,4‐dioxovalerate, diglycolic acid, d‐valine, styrene, 3‐aminopyridine, and hexyl acetoacetate) that triggered termite aggregation. Among them, phenol (1 000 μg/mL), 3‐ethoxypropionic acid (10 μg/mL), ethyl 2,4‐dioxovalerate (1 000 μg/mL), and diglycolic acid (1 000 μg/mL) showed the strongest activities, triggering termite aggregation throughout the 24‐h period. As T. asperellum and T. virens produce different metabolites that trigger aggregation behavior in termites, the mechanisms underlying the interaction between subterranean termites and Trichoderma fungi likely vary. Future studies are needed to test whether these chemicals can attract termites and increase bait consumption.