Rice is the principal food for over half of the population of the world. With its genome size of 430 megabase pairs (Mb), the cultivated rice species Oryza sativa is a model plant for genome research. Here we report the sequence analysis of chromosome 4 of O. sativa, one of the first two rice chromosomes to be sequenced completely. The finished sequence spans 34.6 Mb and represents 97.3% of the chromosome. In addition, we report the longest known sequence for a plant centromere, a completely sequenced contig of 1.16 Mb corresponding to the centromeric region of chromosome 4. We predict 4,658 protein coding genes and 70 transfer RNA genes. A total of 1,681 predicted genes match available unique rice expressed sequence tags. Transposable elements have a pronounced bias towards the euchromatic regions, indicating a close correlation of their distributions to genes along the chromosome. Comparative genome analysis between cultivated rice subspecies shows that there is an overall syntenic relationship between the chromosomes and divergence at the level of single-nucleotide polymorphisms and insertions and deletions. By contrast, there is little conservation in gene order between rice and Arabidopsis.

Streamer-to-filament transition is a general feature of nanosecond discharges at elevated pressure. The transition is observed in different discharges by different groups: in the nanosecond surface dielectric barrier discharges (nSDBDs) in a single shot regime at high pressure (2-15 bar), in the point-to-point or point-to-plane open electrodes discharges at high repetitive frequency (so-called nanosecond repetitive pulsed discharges, NRPDs) at atmospherics pressure. The present paper contains experimental analysis of plasma properties in the filamentary nSDBD: the electrical current, the specific deposited energy, the electron density and the electron temperature were measured for a wide range of pressures and voltages. A model explaining plasma properties in filamentary nanosecond discharges and the role of excited species in streamer-to-filament transition is suggested and discussed.