This experiment was conducted to investigate the effect of the addition of enzymes and microorganisms to broiler feed on productivity, carcass characteristics, intestinal microflora, and feces odor. A total of one-hundred eighty 180 chicks (Ross 308) were randomly assigned to 5 treatments with 3 replications each having 12 birds per pen. The experimental group was divided into 0.1% EZ group (0.1% metallo-protease added to the feed), 0.2% EZ group (0.2% metallo-protease added to the feed), M group (2.0% Bacillus veleznesis CE 100 added to the feed), and MW group (2.0% Bacillus veleznesis CE 100 added to the feed and drinking water). In the results, final body weight, body weight gain, the feed conversion ratio, protein efficiency, and energy efficiency were not significantly different among all treatments in across all periods. Carcass weight, proventriculus, gizzard, heart, small intestine, cecum, and rectum weight were not significantly different among all of the treatments. However the liver weight was significantly higher in the 0.1% EZ group than in the control, M and MW groups (P< 0.05). E. coli was significantly lower in MW than in the control and M (P<0.05), and it was significantly higher in the M than 0.2% EZ and MW (P<0.05). H 2 S emissions in feces was not significantly different among all treatments, but NH 3 emissions was were significantly higher in 0.1% EZ than in MW (P<0.05). In conclusion, the addition of 0.1% of metallo-protease was effective in the development of the liver of broilers.
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