Membranous glomerulonephritis (MGN) is a common cause of idiopathic nephrotic syndrome in adults endstage renal disease in 25% of patients. MYH9 gene polymorphisms have been reported to be associated with several types of renal diseases. The objective of this study was to clarify the relationship between MYH9 gene polymorphisms and the pathogenesis of MGN. We investigated MYH9 gene polymorphisms (rs7078 and rs12107) and their association with MGN susceptibility in 400 Taiwanese individuals (135 MGN patients and 265 healthy controls). The results revealed a statistically significant difference in allele frequency distribution at the rs12107 between MGN patients and the control group (p = 0.04). In addition, individuals with the AA genotype at the rs12107 SNP who become MGN patients may have a higher risk of kidney failure than other MGN patients (adjusted odds ratio: 1.63; 95% confidence interval: 1.08-2.48, p = 0.02). A C-A haplotype was susceptible for development of MGN. Our data show that MYH9 (rs12107) polymorphism may be the underlying cause of MGN; hence the polymorphism examined in this study warrant further investigation.