Background: Phytochemicals are well known to have many important pharmaceutical properties. Heritiera fomes grows in low saline environments. The present study was initiated to explore the biologically beneficial properties of H. fomes. Methods: The crude extracts of H. fomes were extracted in acetone by orbital shaker and concentrated using rota evaporator. Total flavonoid content and Total phenolic content were estimated spectrophotometrically and the in-vitro antioxidant capacity was estimated by DPPH (2,2-diphenyl-1-picrylhydrazyl), Ferric Reducing Antioxidant Power (FRAP) and Hydrogen peroxide (H 2 O 2 ) Scavenging Assays. Anti-microbial activity was determined by Disc diffusion method. Results: Phytochemical screening of acetone extract of H. fomes showed the presence of major classes of phytochemicals like alkaloids, glycosides, flavonoids, saponins, carbohydrates, phenols and sterols in considerable quantity. The total amount of phenolic and flavonoid content was found to be 75.3 mg GAE/g dry weight and 61.3 mg QE/g dry weight respectively. Antioxidant activity of plant extract determined by using different assays like H 2 O 2 radical scavenging, DPPH and FRAP. A positive correlation between all the pairs of antioxidant assays was observed. Further, antimicrobial activity against various pathogens was evaluated by disc diffusion method where the zone of inhibitions were found to be in range from 5± 0.35 to 12 ± 0.50 mm. Conclusion: The study concludes that the plant Heritiera fomes has its effect in scavenging free radicals and has a potential to be a power antioxidant. Several in-vitro studies possess significant antioxidant, antimicrobial activities. The present study plays an important tool for new drug discovery.

The human breast cancer cells (MDAMB-231) were brought from NCCS, Pune. The MDAMB-231 cells were cultured in the laboratory under sterile conditions in the tissue culture flasks. The RPMI-1640 medium provided to the cells which is supplemented with 10% FBS (fetal bovine serum), streptomycin sulphate (100g/ml) and penicillin (100IU/ml). Cells were incubated at 37°C, 5% CO 2 and 100% humidity.