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We previously demonstrated that long-term pretreatment of rat FRTL-5 thyroid cells with TSH or cAMP-generating reagents potentiated IGF-I-dependent DNA synthesis. Under these conditions, cAMP treatment increased tyrosine phosphorylation of a 125-kDa protein (p125) and its association with a p85 regulatory subunit of phosphatidylinositol 3-kinase (p85 PI3K), which were suggested to mediate potentiation of DNA synthesis. This study was undertaken to identify p125 and to elucidate its roles in potentiation of DNA synthesis induced by IGF-I. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis revealed p125 to be a rat ortholog of human XB130, which we named PI3K-associated protein (PI3KAP). cAMP treatment elevated PI3KAP/XB130 mRNA and protein levels as well as tyrosine phosphorylation and interaction with p85 PI3K leading to increased PI3K activities associated with PI3KAP/XB130, supporting the role of PI3KAP/XB130 in DNA synthesis potentiation. Importantly, PI3KAP/XB130 knockdown attenuated cAMP-dependent potentiation of IGF-I-induced DNA synthesis. Furthermore, c-Src was associated with PI3KAP/XB130 and was activated in response to cAMP. Addition of Src family kinase inhibitors, PP1 or PP2, during cAMP treatment abolished tyrosine phosphorylation of PI3KAP/XB130 and its interaction with p85 PI3K. Finally, introduction of PI3KAP/XB130 into NIH3T3 fibroblasts lacking endogenous PI3KAP/XB130 enhanced IGF-I-induced DNA synthesis; however, a mutant Y72F incapable of binding to p85 PI3K did not show this response. Together, these data indicate that cAMP-dependent induction of PI3KAP/XB130, which is associated with PI3K, is required for enhancement of IGF mitogenic activities.

PHNs supported people because of a compelling sense of mission yet it was a great burden. Education about radiation and radiation protection for nursing students and PHNs, two-way communication between PHNs and radiation specialists, long-term support by specialists, and the opportunity for PHNs to share their feelings and experiences is necessary.

Objective To describe the di culties faced by immigrant Chinese mothers caring for their children in Japan and their process of coping with cross-cultural experiences.Methods We conducted semi-structured interviews of 8 Chinese-Japanese mothers living in the Tokyo metropolitan area who had given birth to and were caring for their children in Japan. A qualitative descriptive research method was used. Results We found 3 categories of di culties that these women faced repeatedly 1 Could not imagine how to be a Japanese mother 2 Were confronted by a wall of a di erent culture 3 Lost their sense of self in cross-cultural experiences. We found 1 common category of coping We found 1 category of outcomes from coping with di culties ey found new identities as mothers with both Japanese and Chinese cultural characteristics.Conclusion We found that they coped with their di culties by observational learning and imitation, and that this result resembles the stage theory of culture shock proposed by Pedersen that is, Chinese-Japanese mothers experienced intercultural adjustment and mothering together over time, because caring for their children forced them to acclimate to their new culture.

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