Propolis is a sticky substance that is collected from plants by honeybees. We previously demonstrated that propolins A, B, C, D, E and F, isolated from Taiwanese propolis (TP), could effectively induce human melanoma cell apoptosis and were strong antioxidant agents. In this study, we evaluated TP for free radical scavenging activity by DPPH (1,2-diphenyl-2-picrylhydrazyl). The phenolic concentrations were quantified by the Folin–Ciocalteu method. The apoptosis trigger activity in human melanoma cells was evaluated. TP contained a higher level of phenolic compounds and showed strong capability to scavenge free radicals. Additionally, TP1g, TP3, TP4 and TP7 exhibited a cytotoxic effect on human melanoma cells, with an IC50 of ∼2.3, 2.0, 3.3 and 3.3 μg/ml, respectively. Flow cytometric analysis for DNA fragmentation indicated that TP1g, TP2, TP3 and TP7 could induce apoptosis in human melanoma cells and there is a marked loss of cells from the G2/M phase of the cell cycle. To address the mechanism of the apoptosis effect of TP, we evaluated its effects on induction of apoptosis-related proteins in human melanoma cells. The levels of procaspase-3 and PARP [poly(ADP-ribose) polymerase] were markedly decreased. Furthermore, propolins A, B, C, D, E and F in TP were determined using HPLC. The results indicate that TP is a rich source of these compounds. The findings suggest that TP induces apoptosis in human melanoma cells due to its high level of propolins.

Two new prenylflavanones, propolin A (2) and propolin B (3), were isolated and characterized from Taiwanese propolis. Both compounds were found to have cytotoxic properties against three cancer cell lines. DNA content analyses and DNA fragmentation indicated that propolin A (2) efficiently induced apoptosis in cancer cell lines, but had no effect on the cell cycle program. Furthermore, both propolin A (2) and B (3) are potential antioxidant agents and show strong scavenging effects against most types of free radicals.

Abstract. The ionospheric total electron contents (TEC) in both northern and southern equatorial anomaly regions are examined by using the Global Positioning System (GPS) in Asian area. The TEC contour charts obtained at YMSM (25.2øN, 121.6øE; 14.0øN geomagnetic) and DGAR (7.3øS, 72.4øE; 16.2øS geomagnetic) stations in 1997, solar minimum, are investigated. It is found that the ionospheric crests manifest remarkable seasonal variations. The TEC values on both northern and southern equatorial anomaly crests yield their maximum values during the vernal and autumnal months, but the winter anomaly does not appear in the southern region. Results show that both crests are fully developed around midday in winter, postnoon in equinoxes and late afternoon in summer, and the two crests move significantly equatorward in winter but slightly poleward in summer and autumn. These phenomena can be fully explained by a combined theory of the transequatorial netural wind, the subsolar point, and the auroral equatorward wind.

[1] Six flux transfer events (FTEs) were encountered during MESSENGER's first two flybys of Mercury (M1 and M2). For M1 the interplanetary magnetic field (IMF) was predominantly northward and four FTEs with durations of 1 to 6 s were observed in the magnetosheath following southward IMF turnings. The IMF was steadily southward during M2, and an FTE 4 s in duration was observed just inside the dawn magnetopause followed $32 s later by a 7-s FTE in the magnetosheath. Flux rope models were fit to the magnetic field data to determine FTE dimensions and flux content. The largest FTE observed by MESSENGER had a diameter of $1 R M (where R M is Mercury's radius), and its open magnetic field increased the fraction of the surface exposed to the solar wind by 10-20 percent and contributed up to $30 kV to the cross-magnetospheric electric potential.