CD133 (prominin-1), a 5-transmembrane glycoprotein, has recently been considered to be an important marker that represents the subset population of cancer stem-like cells. Herein we report the isolation of CD133-positive cells (LC-CD133+) and CD133-negative cells (LC-CD133−) from tissue samples of ten patients with non-small cell lung cancer (LC) and five LC cell lines. LC-CD133+ displayed higher Oct-4 expressions with the ability to self-renew and may represent a reservoir with proliferative potential for generating lung cancer cells. Furthermore, LC-CD133+, unlike LC-CD133−, highly co-expressed the multiple drug-resistant marker ABCG2 and showed significant resistance to chemotherapy agents (i.e., cisplatin, etoposide, doxorubicin, and paclitaxel) and radiotherapy. The treatment of Oct-4 siRNA with lentiviral vector can specifically block the capability of LC-CD133+ to form spheres and can further facilitate LC-CD133+ to differentiate into LC-CD133−. In addition, knock-down of Oct-4 expression in LC-CD133+ can significantly inhibit the abilities of tumor invasion and colony formation, and increase apoptotic activities of caspase 3 and poly (ADP-ribose) polymerase (PARP). Finally, in vitro and in vivo studies further confirm that the treatment effect of chemoradiotherapy for LC-CD133+ can be improved by the treatment of Oct-4 siRNA. In conclusion, we demonstrated that Oct-4 expression plays a crucial role in maintaining the self-renewing, cancer stem-like, and chemoradioresistant properties of LC-CD133+. Future research is warranted regarding the up-regulated expression of Oct-4 in LC-CD133+ and malignant lung cancer.

Determining the seismic fracture energy during an earthquake and understanding the associated creation and development of a fault zone requires a combination of both seismological and geological field data. The actual thickness of the zone that slips during the rupture of a large earthquake is not known and is a key seismological parameter in understanding energy dissipation, rupture processes and seismic efficiency. The 1999 magnitude-7.7 earthquake in Chi-Chi, Taiwan, produced large slip (8 to 10 metres) at or near the surface, which is accessible to borehole drilling and provides a rare opportunity to sample a fault that had large slip in a recent earthquake. Here we present the retrieved cores from the Taiwan Chelungpu-fault Drilling Project and identify the main slip zone associated with the Chi-Chi earthquake. The surface fracture energy estimated from grain sizes in the gouge zone of the fault sample was directly compared to the seismic fracture energy determined from near-field seismic data. From the comparison, the contribution of gouge surface energy to the earthquake breakdown work is quantified to be 6 per cent.

The Taiwan Chelungpu‐fault Drilling Project (TCDP) was undertaken in 2002 to investigate the faulting mechanism of the 1999 Taiwan Chi‐Chi earthquake. Hole B penetrated the Chelungpu fault, and recovered core samples from between 948.42 m and 1352.60 m depth. Three zones, marked 1136mFZ, 1194mFZ and 1243mFZ, were recognized in the core samples as active fault‐zones within the Chelungpu fault. Multi‐Sensor Core Logger measurements revealed lower densities and higher magnetic susceptibilities within the black gouge zones in all three fault zones. Even though the fault zone that slipped during the 1999 earthquake has not been identified, higher magnetic susceptibilities indicate that frictional heating has taken place in the Chelungpu fault.

The Taiwan Chelungpu‐fault Drilling Project penetrated three fault zones as the Chelungpu fault system, which slipped during the 1999 Chi‐Chi earthquake, discovering disk‐shaped black material (BM disk) within the middle and lower fault zones in Hole B. The microscopic features of the BM disks indicated that they were pseudotachylytes, and they showed high magnetic susceptibility, possibly the result of intense shearing or high temperature conditions. Inorganic carbon content of the BM disks was low, possibly because of thermal decomposition of carbonate minerals. The high temperatures might be related to frictional heating during the earthquake, implying that the BM disks were produced under intense shearing with frictional heating that reached melting temperature. Because the disks, which provide the only evidence of melting, pre‐date the 1999 earthquake, we concluded that frictional melting did not occur during the earthquake.