Vaccine is one of the major ways of preventing and controlling infectious diseases of humans and animals including birds. The questioner survey was conducted purposively in three selected kebeles, namely Kimmoye, Damotu, and Amaro of Ejere district West Shewa Zone of Oromia Region, Ethiopia from July 2021 to September 2021 in order to assess the knowledge and attitudes of the farmers on the livestock vaccination. A total of 120 respondents were interviewed for this study and face-to-face semi-structured questionnaire survey was employed to collect required data on the livestock vaccination. Descriptive statistics and Chi-square (χ 2 ) were used for the data analyses. In present study, the highest numbers of the farmers (79%) were found to use antibiotics as primary choice to prevent and control livestock diseases. Whereas 21% of the farmers replied on the use of vaccines. Large numbers (45%) of the farmers reared animals both for income and cultural purpose. However, 12% and 43% of the farmers were holding the animals only for culture and income issues, respectively. Additionally, 79% of the farmers in the study area did not take any training on the animal diseases and vaccinations. More than 50% of the farmers did not have good knowledge and positive attitudes toward animal vaccination. Finally, this study revealed, there was lack of awareness among the community about animal diseases and vaccination. Therefore, creating awareness concerning animal diseases and vaccination in the study area is imperative.