Charge movement was studied in the cut twitch fibres of Rana temporaria with a double Vaseline‐gap voltage‐clamp technique. In fibres containing 0.1 mm EGTA, the Iγ hump component of charge movement was either unresolvable or had a brief duration at all potentials. In the steady‐state charge‐voltage (Q–V) plots that were separable into the less voltage dependent (Qβ) and more voltage dependent (Qγ) components, the amount of Qγ was 0.9 ± 0.1 nC μF−1 (mean ±s.e.m., n= 18). Fibres containing 20 mm EGTA showed broad Iγ humps at about −45 mV. The addition of 1.8 mm total Ca2+ to the end‐pools accelerated the rising phase and abbreviated the duration of the hump. I γ humps in fibres containing 0.1 mm EGTA resembled those in intact fibres when recorded at similar low temperatures. These results explain the controversy concerning the variable appearance of Iγ humps in cut fibres.