Abstract. Moringa olifera (drumstick tree) is a medicinal plant commonly used in phytomedicine to cure and prevent diseases in Nigeria. The ethanolic extract of the leave sample showed alkaloid {(9.66 ± 0.20) %}, phenols {(0.75 ± 0.22) %}, flavonoids {(6.86 ± 0.20) %}, saponines {(8.46 ± 0.10) %}, and tannins {(1.10 ± 0.20) %}. The plant also contains nutritional nutrients such as ash {(6.08 ± 0.02) %}, protein {(23.65 ± 0.12)%}, carbohydrates {(57.01 ± 0.01)%}, fats {(2.43 ± 0.01) %}, crude fibre {(6.02 ± 0.13)%}, moisture {(9.50 ± 0.10)%} and energy {(3276 ± 0.14)%}. Antibacterial studies showed that the plant leave successfully inhibited staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Klebisiella pneumonia. Acridine diglycoside was elucidated using Bruker NMR spectroscopy in combination with FT-IR NATRIC and HREIMS (m/z) mass spectral data. This result authenticates the use of the plant leave in the treatment of infections, tumors and as an anti-inflammatory agent