An improvement in colonizing the biting midge Forcipomyia taiwana (Shiraki) was achieved by a new technique that facilitated the rearing of the midges and induced them to mate in the laboratory. At temperature of 15, 20, 25, and 30 degrees C, the development duration of the egg, 4 larval instars, and pupa decreased as temperature increased. Among 7 different diets, the blue green algae, Anabaena sp.Ch3, was the best food for rearing the midges. When the larvae were fed on the blue green algae at 25 degrees C, they needed 12 d to pupate, the pupation rate was 71.4%, the emergence rate was 80.2%, and the average longevity of the male and the female 38.3 and 22.6 d, respectively. When 120 paris were kept in a plastic cage (60 by 60 by 60 cm), swarming and copulation occurred during 0700-0900 and 1700-1800 hours. Swarm occurred throughout the cage and consisted of 10 males. The copulation was performed on the wall and the bottom of the cage, and the average duration was 290 s.

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We studied the distribution of Forcipomyia taiwana (Shiraki) in Taiwan, and found this species almost island-wide. Midge seasonality was studied for 4 yr at 3 sites in Nantou, central Taiwan, to identify the extent and causes of midge population outbreaks. The midge population in 1995 was significantly lower than in 3 other years because several typhoons inundated breeding sites. Maximum populations of F. taiwana occurred in June, July, and August. There was a highly significant correlation between the monthly abundance of F. taiwana and temperature and rainfall. A step-up multiple regression indicated that temperature was the most important factor leading to the outbreaks of F. taiwana. Temperature increases from 15 degrees C to near 30 degrees C will increase the midge abundance.

The purpose of this study was to develop a colorimetric assay for detecting hydrogen peroxide (H2O2) through a combination of using an aryl boronate (AB) derivative and gold nanoparticles (AuNPs). The unique optical property of AuNPs is applied to design a detection probe. The aggregation of AuNPs could be directly observed as a color change by the naked eye. A mannoside‐boronate‐sulfide (MBS) ligand was designed that contains an arylboronate (AB), a mannoside, and a thiol group. The thiol group bonds covalently with the surface of AuNPs to obtain MBS@AuNPs. The mannoside moiety recognizes concanavalin A (Con A), a lectin with four carbohydrate recognition sites that can specifically recognize the non‐reducing end of an α‐D‐mannoside or α‐D‐glucoside structure. The AB structure on MBS first reacts with H2O2 and then inserts an oxygen atom in the B−H bond, which triggers intramolecular electron rearrangement to cleave the covalent bond, resulting in a MBSt mixture. The MBS or MBSt is then modified to citrate‐coated AuNPs (c‐AuNPs) to have MBS@AuNPs or MBSt@AuNPs. When the MBS@AuNPs are incubated with Con A, the Con A recognizes multiple mannosides on the surface of the MBS@AuNPs. Subsequently, the MBS@AuNPs aggregate and the solution's color changes from red to purple, but this color change does not occur in the case of MBSt@AuNPs. The phenomenon can be observed by the naked eye.

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