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In a previous paper, we reported a simple bioassay method for photosynthesis accelerators using wheat protoplasts and an oxygen electrode. With this bioassay method, choline chloride and its analogues were revealed to be photosynthesis accelerators.1} In this paper, we report the photosynthesis acceleration due to TVsubstituted glycine analogues in wheat protoplasts. Wheat {Triticum aestivum: Nourin No. 61) seedlings were grown in Vermiculite for 9 to 12 days in a naturallight Phytotron, which was kept at 25/20°C (day/night). Mesophyll protoplasts were isolated enzymatically by the method of Edwards et al.2) using a combination of cellulase (Onozuka R-10) and pectinase (Macerozyme R-10) purchased from Yakult Biochemical Co., Ltd. The Nsubstituted glycine analogues were synthesized by the method of Kirino et al.3) at the Biochemical Research

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