A total of 640 22-wk-old pullets (Shaver SX 288) housed four birds per cage in 40 experimental units (four cages per unit), were randomly assigned eight experimental diets in a 2 x 2 x 2 factorial arrangement. The treatments consisting of two grain sources (wheat and rye) two levels each of crude enzyme preparation (0 and 0.1% Roxazyme G), and added inorganic phosphorus (0 and 0.105%) were fed for five 4-wk periods. At 42 wk of age, 40 individually caged layers were fed the experimental diets with 0.3% chromic oxide (5 individual birds per treatment) to determine AMEn and available P. Plasma P and Ca were also determined. Egg production, feed intake (FI), egg weight, feed efficiency (FE), and specific gravity of eggs were significantly (P < or = 0.05) affected by the experimental periods. Hens fed wheat-based diets had higher (P < or = 0.05) tibia ash (54.3 vs 52.5%), excreta dry matter (22.0 vs 17.7%), and eggshells with > or = 1.080 specific gravity (93.5 vs 89.9%) than birds fed rye. Enzyme supplementation significantly improved AMEn (P < or = 0.01) and FE by 6.2 and 3%, respectively. Egg production increased numerically from 87.6 to 90.1%. Inorganic P supplementation significantly increased egg production (P < or = 0.01), FI, FE, and AMEn (P < or = 0.05) by 4.4, 2, 3, and 2.8%, respectively, but significantly (P < or = 0.05) decreased the proportion of eggs having a specific gravity > or = 1.080 from 92.8 to 90.6%. The enzyme and inorganic P supplementation had no effect on tibia ash content and total plasma Ca and P. Rye can be used in layer rations yielding satisfactory performance when fed with a fungal crude enzyme preparation high in pentosanase/xylanase activity.

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Body phosphorus homeostasis network allows laying hens to adapt to wide range of changes in dietary phosphorus levels. Phytase hydrolyzes phytate rendering phosphorus and reduces the laying hens' requirements for inorganic phosphate rock. Here, we demonstrate that there is no need to keep large safety margins in dietary phosphorus when hens are fed with phytase. Hy-Line Brown laying hens (n = 504) were randomly assigned to 7 treatments (6 replicates of 12 birds). A corn-soybean meal–based diet, with no inorganic phosphate rock, was formulated to contain 0.12% nonphytate phosphorus ( nPP ), 3.8% calcium, and 2,000 FTU/kg phytase. Inorganic phosphate rock (di-calcium phosphate) was supplemented into the basal diet to create 6 other diets containing 0.17, 0.22, 0.27, 0.32, 0.37, and 0.42% nPP. Levels of calcium carbonate and zeolite powder were adjusted to make sure all the 7 experimental diets contained the same nutrition levels (including calcium and phytase) except nPP. The diets were subjected to laying hens from 29 to 40 wk of age. As a result, when supplemented with 2,000 FTU/kg phytase, extra supplementation of inorganic phosphate rock had no effects ( P > 0.05) on serum phosphorus levels, serum calcium levels, laying performance (laying rate, egg weight, feed intake, feed-to-egg ratio, and unqualified egg rate), egg quality (shell thickness, shell strength, albumen height, yolk color, and Haugh unit), and tibia quality parameters (breaking strength and ash, calcium, and phosphorus contents). Extra supplementation of inorganic phosphate rock linearly increased ( P < 0.01) fecal phosphorus excretion and linearly decreased ( P = 0.032) the apparent metabolizability of dietary phosphorus. While serum hormones and intestine gene expressions were varied within treatments, no consistent changes were found. In conclusion, the supplementation of inorganic phosphate rock (provided 0.05–0.30% extra nPP) to phytase-containing basal diets (2,000 FTU/kg; nPP = 0.12%) provided limited benefits to egg production performance in laying hens from 29 to 40 wk of age. Further investigating the body phosphorus homeostasis would help to understand the nutritional and physiological reasonability of formulating low-phosphorus diets in the laying hen industry.

Dietary phosphorus oversupply wastes non-renewable natural resources and raises environmental concerns in animal agriculture. We hypothesized that laying hens do not need large safety margins for dietary phosphorus because of the existence of fibroblast growth factor 23 (FGF23). In experiment 1, a total of 504 Hy-Line Brown laying hens (40-week-old) were randomly assigned to seven diets (for each diet, six replicates of 12 hens), containing 0.12, 0.17, 0.22, 0.27, 0.32, 0.37, and 0.42% non-phytate phosphorus, respectively, for 15 weeks. In experiment 2, a total of 14 Hy-Line Brown laying hens (40-week-old) were randomly assigned to two diets: (1) phosphorus restricted (n = 7) diet containing 0.14% non-phytate phosphorus, and (2) regular phosphorus (n = 7) diet containing 0.32% non-phytate phosphorus, for 21 days. Laying performance and egg quality were investigated in experiments 1 and 2. Phosphorus excretion and physiological changes were determined in experiment 2. It was found that dietary non-phytate phosphorus levels had no effects (P > 0.05) on laying performance and egg quality in either experiment. In experiment 2, laying hens fed 0.14% non-phytate phosphorus had decreased phosphorus excretion (by 52.6%, P < 0.001) when compared to those fed 0.32% non-phytate phosphorus. In response to the 0.14% non-phytate phosphorus diet, laying hens in experiment 2 exhibited: (1) suppressed calvaria mRNA expressions of FGF23 (by 57.8%, P < 0.001) and fibroblast growth factor receptor 1 (FGFR1, by 52.8%, P = 0.012), (2) decreased serum levels of FGF23 (by 41.7%, P = 0.011) and phosphorus (by 40.3%, P < 0.001), (3) decreased kidney mRNA expressions of FGFR1 (by 66.0%, P = 0.040) and FGFR4 (by 63.3%, P = 0.012) and decreased kidney protein expression of type 2a sodium-phosphorus co-transporter (NPt2a, by 51%, P = 0.025), (4) increased duodenum protein expression of NPt2b (by 45%, P = 0.032), and (5) increased excretion of calcium (by 22.9%, P ≤ 0.024). Collectively, decreasing dietary non-phytate phosphorus by up to 0.12% had no negative effects on egg-production performance but significantly decreased phosphorus excretion in laying hens. The laying hens adjusted to low-phosphorus diets by increasing intestinal NPt2b protein production, which was associated with decreased serum FGF23 concentration. Decreasing dietary non-phytate phosphorus is suggested to laying-hen nutritionists.

Background Dietary supplemental nicotinamide is used to treat hyperphosphatemia in humans. However, the mechanisms of its impact on body phosphorus homeostasis remain unclear. Objective This study was to determine effects and molecular mechanisms of 3 dietary nicotinamide concentrations on body phosphorus homeostasis in laying hens. Methods Hy-Line Brown layers (total = 21; 40 wk old; body weight: 1,876 ± 24 g) were individually housed (n = 7) and fed a corn-soybean meal–based diet supplemented with nicotinamide at 20 (N20), 140 (N140), and 1000 (N1000) mg/kg for 21 d. Serum phosphorus and fibroblast growth factor 23 (FGF23) concentrations, phosphorus and calcium excretion, and mRNA and/or protein of type II sodium-phosphate co-transporters (NPt2a, NPt2ab) and FGF23 and FGF23 receptors were measured in the intestines, calvaria, kidney, and liver. Results Hens in the N1000 group had a 16% lower serum phosphorus concentration and 22% greater phosphorus excretion than those in the N20 or N140 group (P ≤ 0.05). Compared with hens in the N20 group, hens in the N140 and N1000 groups, which did not differ, had 15–21% lower serum FGF23 concentrations, 19–22% greater calcium excretion, 43–56% lower ileum NPT2b protein production, and 1.5- to 1.6-fold greater kidney NPT2a protein production, respectively (all differences at P ≤ 0.05). Conclusions Supplementing high concentrations of nicotinamide in diets for laying hens led to accelerated phosphorus and calcium excretions and decreased serum phosphorus and FGF23 concentrations, which were associated with downregulated intestinal NPt2b protein production. Our findings exclude kidney NPt2a protein production as a primary mechanism for the nicotinamide-induced body phosphorus loss.