Motor imagery and neurofeedback have been proposed as motor training approaches, but their effects on the enhancement of motor performance are still controversial. This study aimed to enhance motor performance using a novel training protocol that combines motor imagery and neurofeedback. Seventeen participants were randomly assigned to the training or control group. The training group received real-time electroencephalography activity feedback relative to the motor imagery of the motor action. The control group reiterated the motor imagery of the intended motor action without feedback. The motor performance of the training group was enhanced significantly more than that of the control group. Subsequently, the proposed training protocol was tested in five elite male tennis players to investigate its applicability to sports activities. The motor performance was enhanced in three of these five professional tennis players. Using this novel training protocol, which combined motor imagery and neurofeedback training, we achieved enhanced motor performance.