Background: Food-borne disease is a major public health problem affecting developed as well as developing countries. Inaccurately treated eggs can be one of its causes. So we designed this study to observe the possibility of transmission of pathogenic Gram-positive bacteria from market eggs to the community.Objectives: To identify different Gram-positive bacteria in eggs and to observe their antimicrobial susceptibility.Materials and Methods: This observational study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka. Shells of 150 eggs collected from different markets of Dhaka city were tested. Bacteria were isolated and identified by culture and relevant biochemical tests.Results: Out of 150 egg shells, 120 (80%) yielded growth of different bacteria. Of them, Staphylococcus spp. were 80 (66.67%), Streptococcus spp. 8 (6.67%), Bacillus subtilis 20 (16.67%) and Bacillus cereus 12 (10%). Out of 80 Staphylococcus spp., 30 (25%) were Staphylococcus aureus and 50 (41.67%) were Staphylococcus saprophyticus. Most of the Gram-positive bacteria were sensitive to ciprofloxacin, ceftriaxone and imipenem. No MRSA and VRSA were found.Conclusion: It can be concluded from this study that Gram-positive bacteria from market eggs may be an important source of infection to the community.J Enam Med Col 2016; 6(1): 15-18

The diagnosis of typhoid fever currently depends on isolation of Salmonella Typhi from blood. The sensitivity of blood culture is very low due to prior antibiotic treatment which is a common practice in Bangladesh. The sensitivity of blood culture also decreases at later stage of the disease. Widal test is the most utilized test in Bangladesh next to blood culture because it is inexpensive, less invasive. But the result of the test is controversial due to false negative & false positive results in some cases.  In this study, a recently introduced polymerase chain reaction-based technique (which has 100% specificity for S. Typhi) was compared with widal test among 80 clinically suspected typhoid fever cases.  Among 80 cases, the respective figures of positivity for PCR & widal test were 70% & 43.75% respectively.  It can be concluded that PCR based technique is more sensitive & much superior to widal for diagnosis of typhoid fever. DOI: http://dx.doi.org/10.3329/bjpp.v30i2.22683 Bangladesh J Physiol Pharmacol 2014; 30(2): 46-50

<p>This study was undertaken to apply<em> Toxoplasma gondii</em> specific IgG avidity test in seropositive pregnant women to differentiate acute and past infection. <em>T. gondii</em> specific IgG avidity test was conducted in 39 seropositive pregnant women and their pregnancy outcomes were observed later on. Out of 39 <em>T. gondii</em> seropositive pregnant women 33 (84%) were only IgG positive and 6 (15.4%) were both IgG-IgM positive. All the IgG positive cases (100%) and 2(33.3%) IgG-IgM positive cases had high avidity antibodies and they gave birth to healthy babies. Rest of the 4 (66.7%) IgG-IgM positive women had low avidity and 50% of them had abortion and 50% gave birth to unhealthy babies. This reveals that the seropositive mothers having high IgG avidity had past infection and no risk of congenital transmission. Seropositive mothers having low IgG avidity had acute infection and so congenital transmission occurred. Presence of<em> T. gondii</em> specific IgG and IgM antibody does not indicate acute infection always. IgG-IgM positive pregnant women should be further evaluated by IgG avidity assay to confirm acute infection.</p><p> </p>