In order to investigate the possible effect of seismic vibration on two-phase flow dynamics and thermal-hydraulics of a nuclear reactor, experimental tests of adiabatic air-water two-phase flow under low-frequency vibration were carried out in this study. An eccentric cam vibration module operated at low motor speed (up to 390rpm) was attached to an annulus test section which was scaled down from a prototypic BWR fuel assembly sub-channel. The inner and outer diameters of the annulus are 19.1mm and 38.1mm, respectively. The two-phase flow operating conditions cover the ranges of 0.03≤<jg> ≤1.46m/s and 0.25≤<jf>≤1.00m/s and the vibration displacement ranges from ±0.8mm to ±22.2mm. Steady-state area-averaged instantaneous and time-averaged void fraction was recorded and analyzed in stationary and vibration experiments. A neural network flow regime identification technique and fast Fourier transformation (FFT) analysis were introduced to analyze the flow regimes and void signals under stationary and vibration conditions. Experimental results reveal possible changes in flow regimes under specific flow and vibration conditions. In addition, the instantaneous void fraction signals were affected and shown by FFT analysis. Possible reasons for the changes include the applied high acceleration and/or induced resonance at certain ports under the specific flow and vibration conditions.