Chemosignals are used by predators to localize prey and by prey to avoid predators. These cues vary between species, but the odor of blood seems to be an exception and suggests the presence of an evolutionarily conserved chemosensory cue within the blood odor mixture. A blood odor component, E2D, has been shown to trigger approach responses identical to those triggered by the full blood odor in mammalian carnivores and as such, is a key candidate as a food/alarm cue in blood. Using a multidisciplinary approach, we demonstrate that E2D holds the dual function of affecting both approach and avoidance behavior in a predator-prey predicted manner. E2D evokes approach responses in two taxonomically distant blood-seeking predators, Stable fly and Wolf, while evoking avoidance responses in the prey species Mouse. We extend this by demonstrating that this chemical cue is preserved in humans as well; E2D induces postural avoidance, increases physiological arousal, and enhances visual perception of affective stimuli. This is the first demonstration of a single chemical cue with the dual function of guiding both approach and avoidance in a predator-prey predicted manner across taxonomically distant species, as well as the first known chemosignal that affects both human and non-human animals alike.

The odor of blood may have both aversive and attractive properties for mammals, depending on the species of the odor donor and the species perceiving the odor. To better understand the informational content of blood odor for a prey species we assessed behavioral responses of male CD-1 mice (n=60) to the odor of blood of same-sex and opposite-sex conspecifics, of a natural predator of mice (cat), and of a herbivore (horse) and an omnivore (human) non-predator of mice. Further, we assessed their behavior towards the mammalian blood odor component trans-4,5-epoxy-(E)-2-decenal which recent studies have shown to be as attractive to mammalian predators as the odor of real blood. A two-compartment test arena was used to record approach/avoidance behavior when the animals were presented with an odor in one compartment and a blank control in the other compartment. We found that both conspecific and heterospecific blood odors elicited significant avoidance behavior in the mice whereas a control odor (n-pentyl acetate) did not. The blood odor component trans-4,5-epoxy-(E)-2-decenal was also significantly avoided and thus appears to play an important role in the perception of mammalian blood odor in this prey species. These results support the notion that mammalian blood odor contains an olfactory warning signal which elicits an adaptive behavioral avoidance response in a prey species, the mouse. Our finding that the mice avoided the mammalian blood odor component trans-4,5-epoxy-(E)-2-decenal to the same degree as the odor of real blood suggests that this volatile compound might be (part of) this warning signal.