Biologically active molecular clones of avian sarcoma virus 17 (ASV 17) contain a replication-defective proviral genome of 3.5 kilobases (kb). The genome retains partial gag and env sequences, which flank a cell-derived putative oncogene of 0.93 kb, termed jun. The jun gene lacks preserved coding domains of tyrosine-specific protein kinases. It also shows no significant nucleic acid homology with other known oncogenes. The probable transformation-specific protein in ASV 17-transformed cells is a 55-kDa gag-jun fusion product.Avian sarcoma virus 17 (ASV 17) was isolated from a spontaneous sarcoma in an adult chicken (1). It screened by plaque hybridization (6) using 32P-labeled long terminal repeat (LTR) or pol-specific probes of avian retroviruses. The LTR-and pol-specific DNA fragments were prepared from pEcoRlD and pHindIII Bam plasmids, respectively. These plasmids were gifts from J. M. Bishop and Nancy Quintrell (University of California, San Francisco). All enzymes employed were purchased from Boehringer Mannheim and were used under the conditions recommended by the supplier.Transfection. Transfection experiments were performed on CEF with recombinant X phage DNAs without the addition of helper virus DNA, according to the method described by Kawai and Nishizawa (7).DNA Sequencing. The DNA fragment encompassing the ASV 17 oncogene was inserted into single-stranded DNA phages M13mpl9 and mpl8. Deletion clones were constructed by using BAL-31 nuclease (8). Both strands were sequenced by the dideoxy chain termination method, using the Klenow fragment of DNA polymerase I (9). Ambiguous regions containing a preponderance of G and C residues were sequenced with reverse transcriptase (10). 2848The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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The primary objective in forensic deoxyribonucleic acid DNA is to develop a useable genetic profile from a piece of evidence to compare with profiles obtained from reference samples from known individuals for the purpose of exclusion.